{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:14:21Z","timestamp":1783512861543,"version":"3.55.0"},"reference-count":66,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1101402"],"award-info":[{"award-number":["2021YFA1101402"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Traumatic brain injury (TBI) is a leading cause of death and disability worldwide, with limited effective treatment strategies. Endogenous neural stem cells (NSCs) give rise to neurons and glial cells throughout life. However, NSCs are more likely to differentiate into glial cells rather than neurons at the lesion site after TBI and the underlying molecular mechanism remains largely unknown. Here, we performed large\u2010scale single\u2010cell transcriptome sequencing of subventricular zone (SVZ) NSCs and NSCs\u2010derived cells in the mouse brain, and provide molecular evidence for previous observations that glial differentiation of NSCs prevails after TBI. In addition, we show that the disrupted neurogenesis following TBI is caused by the reduction of a NSC subcluster (NSC\u20104) expressing the neuronal gene\n                    <jats:italic>Tubb3<\/jats:italic>\n                    . Finally, we demonstrate that the transcriptional factor\n                    <jats:italic>Dlx2<\/jats:italic>\n                    is significantly downregulated in NSC\u20104, and\n                    <jats:italic>Dlx2<\/jats:italic>\n                    overexpression is sufficient to drive NSCs towards neuronal lineage differentiation at the expense of astrocytic lineage differentiation under pro\u2010inflammatory conditions.\n                  <\/jats:p>","DOI":"10.1002\/glia.24671","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T06:14:55Z","timestamp":1736144095000},"page":"1036-1050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Single\u2010Cell\n                    <scp>RNA<\/scp>\n                    Sequencing Uncovers Molecular Features Underlying the Disrupted Neurogenesis Following Traumatic Brain Injury"],"prefix":"10.1002","volume":"73","author":[{"given":"Cong","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Institute of Mental Health and Drug Discovery, Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), School of Mental Health Wenzhou Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang\u2010Hong","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology Chinese Academy of Sciences  Beijing China"},{"name":"Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan\u2010Cheng","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ 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