{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:13Z","timestamp":1762067113063},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T00:00:00Z","timestamp":1097539200000},"content-version":"vor","delay-in-days":4668,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Double\u2010barrelled pH\u2010sensitive microelectrodes were used to measure the intracellular pH (pH<jats:sub>i<\/jats:sub>) of neuropil glial cells and the pH of extracellular spaces (pH<jats:sub>e<\/jats:sub>) within isolated, intact ganglia of the leech <jats:italic>Hirude medicinalis<\/jats:italic>. By application of a weak acid (propionate, 40 mM) or a weak base (ammonium, 20 mM) the total buffer capacity was estimated by changes in glial pH<jats:sub>i<\/jats:sub> and in pH<jats:sub>e<\/jats:sub>. The buffering power of glial cells and in the extracellular spaces was increased by up to threefold in the presence of CO<jats:sub>2<\/jats:sub>\/bicarbonate. The anion exchange inhibitor 4,4\u2010diisothiocyanatostillbene\u20102,2\u2032\u2010disulphonic acid (DIDS, 0.3\u20130.5 mM) reversed this increase in buffering power <jats:italic>both<\/jats:italic> in the glial cells <jats:italic>and<\/jats:italic> in the extracellular spaces. Inhibitors of the carbonic anhydrases reduced the CO<jats:sub>2<\/jats:sub>\/bicarbonate\u2010dependent increase in extracellular buffering power. The results suggest that extracellular H<jats:sup>+<\/jats:sup> buffering dependent upon the availability of bicarbonate is linked to DIDS\u2010sensitive bicarbonate transport across the glial membrane.<\/jats:p>","DOI":"10.1002\/glia.440050107","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:21:52Z","timestamp":1104538912000},"page":"43-47","source":"Crossref","is-referenced-by-count":43,"title":["Evidence for glial control of extracellular pH in the leech central nervous system"],"prefix":"10.1002","volume":"5","author":[{"given":"Joachim W.","family":"Deitmer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(90)90034-E"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(88)90197-2"},{"key":"e_1_2_1_4_1","first-page":"203","article-title":"Stimulation\u2010evoked changes in extracellular pH, calcium and potassium activity in the frog spinal cord","volume":"37","author":"Chvatal A.","year":"1988","journal-title":"Physiol. Bohemoslov."},{"key":"e_1_2_1_5_1","first-page":"R30","article-title":"Contribution of electrogenic Na+\u2010HCO3\n                  \u2212\u2010cotransport to glial H+\u2010buffering in the leech central nervous system","volume":"415","author":"Deitmer J. W.","year":"1990","journal-title":"Pflugers Arch. [Suppl.]"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","unstructured":"Deitmer J. W.(1991) Electrogenic sodium\u2010dependent bicarbonate secretion by glial cells of the leech central nervous system.J. Gen. Physiol.(in press).","DOI":"10.1085\/jgp.98.3.637"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1242\/jeb.91.1.87","article-title":"Measurements of the intracellular potassium activity of Retzius cells in the leech central nervous system","volume":"91","author":"Deitmer J. W.","year":"1981","journal-title":"J. Exp. 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