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The enzymatic activity of the plasma membrane glycoprotein 5\u2032\u2010nucleotidase was also assessed. The cells were obtained from control fetuses (no exposure to ethanol) and from fetuses prenatally exposed to ethanol.<\/jats:p><jats:p>The main findings were: (1) both proliferating and differentiating control astrocytes showed two distinct types of surface con A receptors that could correspond to high\u2010 and low\u2010affinity binding sites; (2) the extent of con A binding was greater in mature than in proliferating control cells; (3) the distribution of con A on cell surface components changed with differentiation; (4) the activity of 5\u2032\u2010nucleotidase showed a substantial increment during the period of differentiation; and (5) prenatal exposure to ethanol clearly decreased the ability of astrocytes to bind con A, altered the surface distribution of the receptors for this lectin, and decreased the activity of 5\u2032\u2010nucleotidase. These effects were more marked in proliferating cells.<\/jats:p><jats:p>In conclusion, it is shown that the extent of con A labeling and the activity of 5\u2032\u2010nucleotidase in astrocytes are dependent on the stage of cell differentiation and that prenatal exposure to ethanol alters the plasma membrane structure of these cells during development.<\/jats:p>","DOI":"10.1002\/glia.440050110","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:21:52Z","timestamp":1104538912000},"page":"65-74","source":"Crossref","is-referenced-by-count":14,"title":["Prenatal exposure to ethanol alters plasma membrane glycoproteins of astrocytes during development in primary culture as revealed by concanavalin a binding and 5\u2032\u2010nucleotidase activity"],"prefix":"10.1002","volume":"5","author":[{"given":"Jaime","family":"Renau\u2010Piqueras","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo","family":"Guerri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Burgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulino","family":"De Paz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana","family":"Saez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Mayordomo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1037\/0033-2909.90.3.564"},{"key":"e_1_2_1_3_1","first-page":"421","article-title":"Consumption of alcohol during pregnancy: A review of effects on growth and development of offspring","volume":"54","author":"Abel E. 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