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This paper reports on the characterization of olfactory nerve glia in dissociated cell cultures of newborn rat nasal mucosal tissues. The predominant type of glial cell resembled Schwann cells and immunostained for the S\u2010100 protein, found in all glial cell types; glial fibrillary acidic protein (GFAP), found in astrocytes and nonmyelinating Schwann cells; and showed binding of 217C, a monoclonal Schwann\u2010cell marker that binds to the low\u2010affinity NGF receptor in glioma cells. They were negative for A2B5. The Schwann\u2010cell\u2010like olfactory glia changed morphology upon culturing in serum\u2010free medium, with further shape changes after plating on laminin. Plating on laminin increased cell numbers. A second population, found only after GFAP\u2010immunostaining, was astrocyte\u2010like in morphology and represented approximately 10 percent of all glial cells. These were S\u2010100\u2010, A2B5\u2010, and 217C\u2010negative, a unique glial cell immunological profile. At low dilutions of anti\u2010GFAP (1\/10,000), or with weak fluorescent second ary antibodies, astrocyte\u2010like glia were immunostained but Schwann\u2010cell\u2010like glia were not detectable. Astrocyte\u2010like glia were not an artifact of the dissection, since they were detectable in tissue sections of newborn\u2010rat olfactory nerves immunostained with a low dilution of anti\u2010GFAP. The presence of two types of glial cell in culture suggests similarities between olfactory glia and entric glia. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.440050405","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:23:59Z","timestamp":1104539039000},"page":"269-284","source":"Crossref","is-referenced-by-count":132,"title":["The olfactory nerve contains two populations of glia, identified both in vivo and in vitro"],"prefix":"10.1002","volume":"5","author":[{"given":"Sarah K.","family":"Pixley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"457","volume-title":"Synaptic Plasticity","author":"Aguayo A. 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