{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:14:53Z","timestamp":1790428493768,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T00:00:00Z","timestamp":1097539200000},"content-version":"vor","delay-in-days":4271,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two types of glial cells occur in the retina, M\u00fcller cells and astrocytes. These cells share several structural features such as extending endfeet onto blood vessels of the retina. Retinal vessels express a tight blood\u2010retinal barrier which is comparable to the blood\u2010brain barrier (BBB) of the CNS. While astrocytes have been implicated in the induction of the BBB, the role of M\u00fcller cells in the blood\u2010retinal barrier is unknown. To determine if M\u00fcller cells are capable of influencing vascular permeability, we have prepared M\u00fcller cells that are free of astrocytes and transplanted them to a peripheral target, the anterior eye chamber. M\u00fcller cells were identified 2 weeks to 3 months after injection and were predominantly localized within the connective tissue of the ciliary body. The M\u00fcller cells occurred as dense clusters of cells closely associated with ciliary blood vessels. The ciliary vessels adjacent to M\u00fcller cells were freely permeable to circulating horseradish peroxidase (HRP), suggesting that M\u00fcller cells did not induce tight barrier properties from these leaky peripheral vessels. In contrast, cortical astrocytes injected into the anterior eye chamber preferentially formed a monolayer on the anterior surface of the iris, a region known to contain blood vessels that are impermeable to circulating tracers (e.g., Raviola, Exp Eye Res [Suppl] 25:27, 1977). M\u00fcller cells were rarely associated with the iris and the few cells that were present were located deep within the iris stroma rather than on the surface. The behaviour of guinea pig M\u00fcller cells transplanted to the anterior eye chamber contrasts sharply with that of cortical astrocytes in terms of: (1) the ocular compartment to which M\u00fcller cells migrate; (2) the tissue invasiveness of the cells; and (3) the degree of permeability of blood vessels adjacent to transplanted cells. The results of this study emphasize the functional distinctness of the two types of retinal glia and suggest that M\u00fcller cells from guinea pig retina may not be active in modifying the permeability properties of peripheral blood vessels, a function that has been suggested for astrocytes.<\/jats:p>","DOI":"10.1002\/glia.440070205","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:46:11Z","timestamp":1104540371000},"page":"158-169","source":"Crossref","is-referenced-by-count":19,"title":["Functional properties of retinal M\u00fcller cells following transplantation to the anterior eye chamber"],"prefix":"10.1002","volume":"7","author":[{"given":"Rochelle K.","family":"Small","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brynmor A.","family":"Watkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Munro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490270319"},{"key":"e_1_2_1_3_1","first-page":"593","article-title":"The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues","volume":"42","author":"Barclay A. N.","year":"1981","journal-title":"Immunology"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(79)90106-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713688409000819"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00237966"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.10.4913"},{"key":"e_1_2_1_8_1","first-page":"1321","article-title":"M\u00fcller cell expression of glial fibrillary acidic protein after genetic and experimental photoreceptor degeneration in the rat retina","volume":"25","author":"Eisenfeld A. J.","year":"1984","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_1_9_1","first-page":"1363","article-title":"Accumulation of glial fibrillary acidic protein in M\u00fcller retinal glia during retinal degeneration","volume":"29","author":"Ekstr\u00f6m P.","year":"1988","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440020409"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(90)90063-Z"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.1.171"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/325253a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/31.7.6406590"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.60.1.316"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1242\/jeb.95.1.35","article-title":"Membrane structure in mammalian astrocytes: A review of freeze\u2010fracture studies in adult, developing, reactive and cultured astrocytes","volume":"95","author":"Landis D. M. D.","year":"1981","journal-title":"J. Exp. Biol."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(88)90068-1"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(88)90119-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.7190"},{"key":"e_1_2_1_20_1","first-page":"4900","article-title":"Effects of mycophenolic acid on detection of glial filaments in human and rat astrocytoma cultures","volume":"47","author":"Lipsky R. H.","year":"1987","journal-title":"Cancer Res."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.85.3.890"},{"key":"e_1_2_1_22_1","first-page":"137","article-title":"Monoclonal antibodies specific to vimentin","volume":"34","author":"Osborn M.","year":"1984","journal-title":"Eur. J. Cell Biol."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/280688a0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.8.2709"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(77)80009-2"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440020406"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(90)90043-A"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713689509000004"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)91618-X"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90138-9"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902400203"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440010109"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903050405"},{"key":"e_1_2_1_34_1","first-page":"219","article-title":"The structure and development of the rat retina: An immunofluorescence microscopical study using antibodies specific for intermediate filament proteins","volume":"30","author":"Shaw G.","year":"1983","journal-title":"Eur. J. Cell Biol."},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440040507"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9394(71)90577-0"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(81)90477-2"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(81)90382-1"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902550104"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.07-10-03293.1987"},{"key":"e_1_2_1_41_1","first-page":"434","article-title":"Comparative study of the fine structure of retinal M\u00fcller cells in various vertebrates","volume":"12","author":"Uga S.","year":"1973","journal-title":"Invest. Ophthalmol."},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00330918"},{"key":"e_1_2_1_43_1","first-page":"892","article-title":"Immunocytochemical characteristics of M\u00fcller cells cultured from adult rabbit retina","volume":"6","author":"Wakakura M.","year":"1988","journal-title":"Inv. Ophthal. Vis. Sci."},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/332834a0"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(87)90268-0"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440010403"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.440070205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.440070205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T10:16:20Z","timestamp":1698228980000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.440070205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2]]}},"alternative-id":["10.1002\/glia.440070205"],"URL":"https:\/\/doi.org\/10.1002\/glia.440070205","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2]]}}}