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Using a mouse model of Parkinson's disease, in which FGF\u20102 has been shown to exert marked neuroprotection of nigrostriatal dopaminergic neurons, we have studied striatal levels of glial fibrillary acidic protein (GFAP), an established marker for astrocytes, and the distribution and morphologies of GFAP\u2010immunoreactive cells following treatments with the neurotoxic drug 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine (MPTP), the growth factor FGF\u20102, and the non\u2010trophic control protein cytochrome C (cyt C). Systemic injections of MPTP (30 mg\/kg) on 3 consecutive days, which we have previously shown to cause profound and long\u2010lasting damage to the nigrostriatal system, induced an approximate 20% transient increase in striatal GFAP, determined by enzyme\u2010linked immunosorbent assay (ELISA), 1 day after the final MPTP injection (= day 4), with subsequent normalization at day 7, which lasted until the end of the experiment (day 18). Morphologically, MPTP elicited a marked increase in number, size, arborization, and stainability of GFAP\u2010immunoreactive cells at day 4 in a striatal area adjacent to the corpus callosum, which was evaluated throughout all experiments. Even on day 18, astrocytes were still apparently larger and more branched than in unlesioned controls. Administration of 4 \u03bcg of either FGF\u20102 or cyt C (soaked into a piece of Gelfoam unilaterally to the right striatum in either MPTP\u2010 or saline\u2010injected controls) increased striatal GFAP levels bilaterally about 2\u2010 to 2.5\u2010fold at 14 days, when FGF\u20102 showed marked protection of dopaminergic parameters. Likewise, GFAP immunocytochemistry revealed increased numbers of intensely immunoreactive astrocytes under any experimental situation. Differences in the morphologies of astrocytes in FGF\u20102\u2010 and cyt C\u2010treated animals were very subtle and only noted at greater distances away from the site of application of the factors. We conclude that FGF\u20102, a potent neurotrophic factor for the neurotoxically lesioned nigrostriatal system, does not cause a marked astrogliotic reaction, which might be expected from previous in vitro and in vivo studies in other neural systems. This may limit concerns regarding potential applicability of FGF\u20102 to the parkinsonian striatum. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.440110107","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:48:07Z","timestamp":1104540487000},"page":"47-56","source":"Crossref","is-referenced-by-count":30,"title":["FGF\u20102 in the MPTP model of parkinson's disease: Effects on astroglial cells"],"prefix":"10.1002","volume":"11","author":[{"given":"D\u00f6rte","family":"Otto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Unsicker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901800412"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(81)90052-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/332360a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(83)90031-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90040-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90530-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(81)90272-7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.22.10237"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(87)90260-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1976.tb00488.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/25.6.69656"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)90362-8"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_15_1","first-page":"471","article-title":"The proliferation of astrocytes around a needle wound in the rat brain","volume":"106","author":"Cavanagh J. 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