{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T09:27:07Z","timestamp":1779355627363,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T00:00:00Z","timestamp":1097539200000},"content-version":"vor","delay-in-days":3452,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[1995,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Corpora amylacea (CA) are cytoplasmic inclusions that accumulate in human brain in the course of normal aging, and to an even greater extent, in Alzheimer's disease and other neurodegenerative conditions. In senescent and Alzheimer\u2010diseased human brains, astrocytes in limbic and periventricular regions exhibit red autofluorescent inclusions, homologous to Gomori\u2010positive astrocyte granules previously described in the brains of aging rodents and other vertebrates. We have shown that Gomori inclusions in situ and in culture are derived from autophagocytosed mitochondria exhibiting iron\u2010mediated peroxidase activity. In the human brain, the autofluorescent inclusions share many properties with CA. Both types of inclusion progressively accumulate in periventricular regions with advancing age, are largely astrocytic in origin, and contain various heat shock proteins and ubiquitin. Using histochemistry in conjunction with confocal microscopy, we demonstrated that both CA and the red autofluorescent granules exhibit non\u2010enzymatic peroxidase activity and an affinity for CAH and PAS. The only major divergent histochemical feature between the Gomori\u2010positive astrocyte granules and CA is the presence of orange\u2010red autofluorescence in the former and the absence of endogenous fluorescence in the latter. On the basis of numerous shared topographic and histochemical features, we hypothesized that CA are largely derived from autofluorescent (Gomori\u2010positive) astrocyte granules which reside in periventricular regions of the senescent CNS. Immunofluorescent labeling and laser scanning confocal microscopy demonstrated consistent colocalization of the mitochondrial proteins, sulfite oxidase, and heat shock protein 60, to both CA and the autofluorescent astroglial inclusions. In addition, both CA and the autofluorescent astrocyte granules exhibit staining for DNA which colocalizes to mitochondrial antigens and therefore likely represents mitochondrial nucleic acid in dual\u2010labeled preparations. These observations suggest that a) Gomori\u2010positive astrocyte granules in human brain are homologous to those described in rodents, b) Gomori\u2010positive granules may be structural precursors of CA in senescent human brain, and c) in the aging human brain, degenerate mitochondria within periventricular astrocytes give rise to autofluorescent cytoplasmic granules and corpora amylacea. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.440140108","type":"journal-article","created":{"date-parts":[[2004,12,31]],"date-time":"2004-12-31T13:52:07Z","timestamp":1104501127000},"page":"55-64","source":"Crossref","is-referenced-by-count":59,"title":["Mitochondrial constituents of corpora amylacea and autofluorescent astrocytic inclusions in senescent human brain"],"prefix":"10.1002","volume":"14","author":[{"given":"Hyman M.","family":"Schipper","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soriba","family":"Ciss\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"405","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"1989"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00432727"},{"key":"e_1_2_1_4_1","first-page":"334","article-title":"Parasynaptic corpora amylacea in the striatum","volume":"105","author":"Averback P.","year":"1981","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/92.3.663"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91516-4"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092400313"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00965562"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227716"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91125-C"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001150309"},{"key":"e_1_2_1_12_1","first-page":"1","article-title":"Molecular chaperones","volume":"1","author":"Ellis R. J.","year":"1991","journal-title":"Semin. Cell Biol."},{"key":"e_1_2_1_13_1","first-page":"1314","article-title":"Neuropathology of olfactory system in Alzheimer's disease and normal aging","volume":"12","author":"Fleming P. D.","year":"1986","journal-title":"Soc. Neurosci. Abst."},{"key":"e_1_2_1_14_1","first-page":"157","article-title":"Corpora amylacea increased in Alzheimer's disease","volume":"37","author":"Fleming P. D.","year":"1987","journal-title":"Neurology (Suppl 1), Abstr."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1971.00490060017002"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(85)90024-7"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/14.9.641"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00234404"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1967.00470240003001"},{"key":"e_1_2_1_20_1","first-page":"1087","article-title":"Diagnosis of Alzheimer's disease","volume":"42","author":"Katchaturian Z. S.","year":"1985","journal-title":"Arch. Neurol."},{"key":"e_1_2_1_21_1","first-page":"9","article-title":"Studies on the Gomori\u2010positive glial cells in rats of various ages","volume":"18","author":"Koritsanszky S.","year":"1967","journal-title":"Acta. Biol. Hung."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90611-2"},{"key":"e_1_2_1_23_1","first-page":"135","article-title":"Gomori\u2010positive glia in the mouse: Post\u2010natal development and topographical distribution","volume":"20","author":"Maksymowicz K.","year":"1972","journal-title":"Folia Biol. (Krakow)"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/40.12.1453006"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb09813.x"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)90754-B"},{"key":"e_1_2_1_27_1","first-page":"223","article-title":"On the Gomoriphil findings other than the neurosecretory system in the observation of the hypothalamophypophysial system","volume":"8","author":"Noda H.","year":"1959","journal-title":"Gumma J. Med. Sci."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.3.1298"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685376"},{"key":"e_1_2_1_30_1","first-page":"261","article-title":"Histochemical detection of polysaccharides in \u201ccorpora amylacea\u201d of human prostates","volume":"14","author":"Pasqualucci E. A. M.","year":"1968","journal-title":"Ann. Histochem."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(91)90033-T"},{"key":"e_1_2_1_32_1","first-page":"241","volume-title":"Molybdenum and Molybdenum\u2010Containing Enzymes","author":"Rajagopalan K. V.","year":"1980"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-196501000-00003"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/103.2.315"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440040404"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod25.2.413"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1163"},{"key":"e_1_2_1_38_1","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1523\/JNEUROSCI.11-07-02170.1991","article-title":"Catechol oxidation by peroxidase\u2010positive astrocytes in primary culture: An electron spin resonance study","volume":"11","author":"Schipper H. M.","year":"1991","journal-title":"J. Neurosci."},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90273-E"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/39.8.1649853"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199307000-00007"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(90)90066-8"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(71)90488-4"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687554"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(90)90010-D"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1990.tb08858.x"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00688149"},{"key":"e_1_2_1_48_1","unstructured":"WangX. ManganaroF. Schipper H. M. A cellular stress model for the sequestration of redox\u2010active glial iron in the aging and degenerating nervous system.J. Neurochem in press."},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901010204"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)75018-4"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00684544"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.440140108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.440140108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T21:39:54Z","timestamp":1695850794000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.440140108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,5]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,5]]}},"alternative-id":["10.1002\/glia.440140108"],"URL":"https:\/\/doi.org\/10.1002\/glia.440140108","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,5]]}}}