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Using the in situ patch\u2010clamp technique, kainate\u2010 and AMPA\u2010induced conductance changes of glial precursor cells in the rat corpus callosum were investigated to study these aspects. Precursor cells were identified by their voltage\u2010gated currents and were easily discernable from astrocytes and oligodendrocytes. Kainate induced two overlying effects in these cells: the activation of a cationic current and the block of potassium conductances. Cesium in the pipette solution blocked potassium conductances nearly completely and the ionic profile of the kainate\u2010induced cationic current could be studied in detail. Full replacement of the sodium in the bath by calcium resulted only in a small kainate\u2010induced (calcium) inward current flow, but the kainate\u2010induced outward current carried by Cs<jats:sup>+<\/jats:sup> was less affected reflecting a weak calcium permeability. The kainate response could be blocked by 6,7\u2010dinitroquinoxaline\u20102,3\u2010dione (DNQX) and millimolar zinc concentrations. Co\u2010application of microinolar concentrations of zinc slightly enhanced the kainate\u2010induced current, while Evans Blue was without any significant effect. Cyclothiazide increased the kainate response by a factor of x6 while concanavalin A did not enlarge it. The AMPA\u2010induced current was amplified by a factor of X39 by cyclothiazide.<\/jats:p><jats:p>The present data suggested the expression of weakly calcium\u2010permeable AMPA receptors on glial precursor cells in the rat corpus callosum. Only a small fraction of the agonist\u2010induced current could be seen without the appropriate blockers of receptor desensitization. An additional expression of kainate\u2010preferring glutamate receptors could not be shown. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.440140205","type":"journal-article","created":{"date-parts":[[2004,12,31]],"date-time":"2004-12-31T13:44:07Z","timestamp":1104500647000},"page":"101-114","source":"Crossref","is-referenced-by-count":30,"title":["AMPA\u2010type glutamate receptors in glial precursor cells of the rat corpus callosum: Ionic and pharmacological properties"],"prefix":"10.1002","volume":"14","author":[{"given":"Thomas","family":"Berger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90145-H"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","unstructured":"Backus K. 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