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Nevertheless, little is known about how A\u03b2 affects OL differentiation and myelination in\u00a0vivo, and the underlying molecular mechanisms. In this study, we explored the effects of a brain intraventricular injection of A\u03b2 on OLs and myelin in the developing spinal cord of zebrafish larvae. Using quantitative fluorescent in\u00a0situ RNA hybridization assays, we demonstrated that A\u03b2 altered <jats:italic>myrf<\/jats:italic> and <jats:italic>mbp<\/jats:italic> mRNA levels and the regional distribution of <jats:italic>mbp<\/jats:italic> during larval development, suggesting an early differentiation of OLs. Through live imaging of <jats:italic>Tg<\/jats:italic>(<jats:italic>myrf:mScarlet<\/jats:italic>) and <jats:italic>Tg<\/jats:italic>(<jats:italic>mbpa:tagRFP<\/jats:italic>) zebrafish lines, both crossed with <jats:italic>Tg<\/jats:italic>(<jats:italic>olig2:EGFP<\/jats:italic>), we found that A\u03b2 increased the number of <jats:italic>myrf<\/jats:italic>\n<jats:sup>+<\/jats:sup> and <jats:italic>mbp<\/jats:italic>\n<jats:sup>+<\/jats:sup> OLs in the dorsal spinal cord at 72 hpf and 5 dpf, respectively, without affecting total cell numbers. Furthermore, A\u03b2 also increased the number of Sox10<jats:sup>+<\/jats:sup>cells, myelin sheaths per OL, and the number of myelinated axons in the dorsal spinal cord at 8 dpf compared to vehicle\u2010injected control animals. Interestingly, the treatment of A\u03b2\u2010injected zebrafish with the pan\u2010PKC inhibitor G\u00f66983 restored the aforementioned alterations in OLs and myelin to control levels. Altogether, not only do we demonstrate that A\u03b2 induces a precocious oligodendroglial differentiation leading to dysregulated myelination, but we also identified PKC as a key player in A\u03b2\u2010induced pathology.<\/jats:p>","DOI":"10.1002\/glia.70015","type":"journal-article","created":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T06:29:21Z","timestamp":1742020161000},"page":"1437-1451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Amyloid\u2010\u03b2 Dysregulates Oligodendroglial Lineage Cell Dynamics and Myelination via <scp>PKC<\/scp> in the Zebrafish Spinal Cord"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1232-9404","authenticated-orcid":false,"given":"Uxue","family":"Balantzategi","sequence":"first","affiliation":[{"name":"Department of Neurosciences University of the Basque Country (UPV\/EHU)  Leioa Spain"},{"name":"Achucarro Basque Center for 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