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We tested the hypothesis that signals sent by developing astrocytes to developing neurons modulate dendritic development in\u00a0vivo. First, we altered neuronal development by exposing neonatal (third trimester\u2010equivalent) mice to ethanol, which increased dendritic arborization in hippocampal pyramidal neurons. We next assessed concurrent changes in the mouse astrocyte translatome by translating ribosomal affinity purification (TRAP)\u2010seq. We followed up on ethanol\u2010inhibition of astrocyte\n                    <jats:italic>Chpf2<\/jats:italic>\n                    and\n                    <jats:italic>Chsy1<\/jats:italic>\n                    gene translation because these genes encode biosynthetic enzymes of chondroitin sulfate glycosaminoglycan (CS\u2010GAG) chains (extracellular matrix components that inhibit neuronal development and plasticity) and have not been explored before for their roles in dendritic arborization. We report that\n                    <jats:italic>Chpf2<\/jats:italic>\n                    and\n                    <jats:italic>Chsy1<\/jats:italic>\n                    are enriched in astrocytes, and their translation is inhibited by ethanol, which also reduces the levels of CS\u2010GAGs measured by Liquid Chromatography\/Mass Spectrometry. Finally, astrocyte\u2010conditioned medium derived from\n                    <jats:italic>Chfp2<\/jats:italic>\n                    \u2010silenced astrocytes increased neurite length and branching of hippocampal neurons in\u00a0vitro, mechanistically linking changes in CS\u2010GAG biosynthetic enzymes in astrocytes to altered neuronal development. These results demonstrate that CS\u2010GAG biosynthetic enzymes in astrocytes regulate dendritic arborization in developing neurons and are involved in ethanol\u2010induced altered neuronal development.\n                  <\/jats:p>","DOI":"10.1002\/glia.70020","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T09:24:50Z","timestamp":1744017890000},"page":"1589-1607","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Astrocyte Extracellular Matrix Modulates Neuronal Dendritic Development"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9599-5308","authenticated-orcid":false,"given":"Joel G.","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Department of Behavioral Neuroscience Oregon Health &amp; Science University  Portland Oregon USA"},{"name":"VA Portland Health Care System  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Margolies","sequence":"additional","affiliation":[{"name":"Department of Behavioral Neuroscience Oregon Health &amp; 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