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There is also growing appreciation for their role in directly modulating neuronal excitability and information transfer. Recently, we reported that astrocytes in the retina exhibit an array of neuronal\u2010associated microstructural motifs whose structure and placement suggest roles in monitoring neuronal electrical activity or direct modulation of excitability. In this study, we record whole\u2010cell patch clamp responses of astrocytes in intact retina to both light and voltage step as a precursor to studying the detailed physiology of individual microstructural motifs. Retinal astrocytes exhibit small amplitude, graded depolarization to both light ON and OFF stimuli with waveforms that closely resemble those of M\u00fcller glial endfeet, from which we also recorded. Depolarization is due to potassium influx, with the major source likely being focal release from M\u00fcller endfeet onto astrocyte soma. Both macroglia additionally share current\u2013voltage relationships and exhibit stimulus\u2010dependent changes in ionic permeability. The results suggest a pathway of communication from M\u00fcller cells to astrocytes that could support broader retinal modulation beyond potassium spatial buffering.<\/jats:p>","DOI":"10.1002\/glia.70022","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T06:45:07Z","timestamp":1745390707000},"page":"1520-1534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Potassium\u2010Dependent Coupling of Retinal Astrocyte Light Response to M\u00fcller Glia"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9304-6013","authenticated-orcid":false,"given":"Joseph Matthew","family":"Holden","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Vanderbilt University Medical Center  Nashville Tennessee USA"},{"name":"Vanderbilt Neuroscience Graduate Program Vanderbilt University  Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9436-8786","authenticated-orcid":false,"given":"Andrew M.","family":"Boal","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Vanderbilt University Medical Center  Nashville Tennessee USA"},{"name":"Vanderbilt Neuroscience Graduate Program Vanderbilt University  Nashville Tennessee USA"},{"name":"University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4512-6355","authenticated-orcid":false,"given":"Lauren Katie","family":"Wareham","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Vanderbilt University Medical Center  Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8475-9959","authenticated-orcid":false,"given":"David John","family":"Calkins","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Vanderbilt University Medical Center  Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1174331"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609225103"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2017.03.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.966425"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900463"},{"key":"e_1_2_9_7_1","first-page":"3316","article-title":"Human M\u00fcller Glial Cells: Altered Potassium Channel Activity in Proliferative Vitreoretinopathy","volume":"40","author":"Bringmann A.","year":"1999","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2006.05.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018525222826"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77087-6"},{"key":"e_1_2_9_11_1","unstructured":"Clark B. 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