{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:17:39Z","timestamp":1791577059020,"version":"4.3.4"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T00:00:00Z","timestamp":1762128000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A15150117"],"award-info":[{"award-number":["2023A15150117"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571182"],"award-info":[{"award-number":["81571182"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870982"],"award-info":[{"award-number":["81870982"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071386"],"award-info":[{"award-number":["82071386"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271393"],"award-info":[{"award-number":["82271393"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471417"],"award-info":[{"award-number":["82471417"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>RhoA is well known as a key molecular switch for cytoskeleton remodeling, and previous studies reveal RhoA plays a pivotal role in Schwann cell myelination which is highly dependent on the dynamics regulation of the actin and microtubule cytoskeleton. Existing evidence indicates RhoA modulates myelination and other biofunctions by targeting actin filament turnover; however, the role of RhoA in microtubule dynamics remains unknown. Herein, Bulk mRNA sequencing and bioinformatic analysis enriched microtubule dynamics\u2010related ontology terms in RhoA knockout Schwann cells, and identified that microtubules contribute to RhoA deficiency\u2010caused hypomyelination. Both in\u00a0vivo and in\u00a0vitro experiments demonstrated that genetic ablation or pharmacological inhibition of RhoA attenuates microtubule dynamics in Schwann cells, whereas activated RhoA overexpression or RhoA agonist enhances the microtubule dynamics. RhoA conditional knockout (cKO) in Schwann cells led to hypomyelination, dysmyelination and nerve functional deficits in mice. Mechanistically, the present study identified CDK2 as a crucial mediating molecule for RhoA regulating microtubule dynamics. CDK2 overexpression could reverse the reduced microtubule dynamics, hypomyelination and motor deficits in RhoA cKO mice. Furthermore, RhoA modulating CDK2 is dependent on YAP\/TEAD signaling, and the ASPM\/p60\u2010Katanin axis mediates the role of CDK2 in controlling microtubule dynamics. Collectively, this study uncovered a novel RhoA\/YAP1\/TEAD3\/CDK2\/ASPM\/p60\u2010Katanin axis in regulating microtubule dynamics during Schwann cell myelination, which indicates that this pathway may be utilized as new targets for repairing congenital hypomyelination\/dysmyelination neuropathy or peripheral nerve injury.<\/jats:p>","DOI":"10.1002\/glia.70093","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:50:39Z","timestamp":1762163439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>RhoA<\/scp>\n                    Enhances Schwann Cell Microtubule Dynamics and Myelination via a\n                    <scp>YAP1<\/scp>\n                    \/\n                    <scp>TEAD3<\/scp>\n                    \/\n                    <scp>CDK2<\/scp>\n                    \/\n                    <scp>ASPM<\/scp>\n                    \/p60\u2010Katanin Axis"],"prefix":"10.1002","volume":"74","author":[{"given":"Xinrui","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingmin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huqian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"He","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haowen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinkun","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7885-2903","authenticated-orcid":false,"given":"Jiasong","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, National Demonstration Center for Experimental Education, School of Basic Medical Sciences; Department of Neurosurgery Institute of Brain Diseases, Nanfang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong Province Key Laboratory of Psychiatric Disorders  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910785"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.05.013"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-024-04576-9"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000109858"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2006.12.008"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233651"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9763"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac124"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac402"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cdev.2024.203993"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b00683"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.4103\/NRR.NRR\u2010D\u201024\u201000081"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2014.00338"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.58591"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.261522"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.91719"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20107"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.87394"},{"key":"e_1_2_10_20_1","first-page":"E2443","article-title":"NAD+ and SIRT3 Control Microtubule Dynamics and Reduce Susceptibility to Antimicrotubule Agents","volume":"111","author":"Harkcom W. T.","year":"2014","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awad413"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23532"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3511"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41393-025-01064-2"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01268092"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.07.008"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.2c00189"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.373657"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.320997"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24365"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.926914"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/769412"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/35087035"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000542"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4316"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1803875115"},{"key":"e_1_2_10_37_1","first-page":"831","article-title":"A Simple Click by Click Protocol to Perform Docking: AutoDock 4.2 Made Easy for Non\u2010Bioinformaticians","volume":"12","author":"Rizvi S. M.","year":"2013","journal-title":"EXCLI Journal"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02531"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041360"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2021.12.006"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107026200"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2023.102481"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2021.08.014"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz151"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00437"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010230"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.016"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-03-01797.1995"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2017.00414"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-024-07204-5"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-26077"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.12.006"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-9733-5"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2018.06.013"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02292-y"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.175955"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2024.04.012"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-021-02607-3"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.70093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.70093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.70093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T13:57:23Z","timestamp":1776434243000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.70093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,3]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/glia.70093"],"URL":"https:\/\/doi.org\/10.1002\/glia.70093","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,3]]},"assertion":[{"value":"2025-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70093"}}