{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T14:48:04Z","timestamp":1776437284307,"version":"3.51.2"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["STE 552\/5"],"award-info":[{"award-number":["STE 552\/5"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SE 774\/6"],"award-info":[{"award-number":["SE 774\/6"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gray matter NG2 glia constitute a heterogeneous population of cells whose functions remain incompletely understood. In the hippocampus, Schaffer collaterals activate AMPA receptors (AMPARs) in NG2 glia, giving rise to small excitatory post\u2010synaptic currents (EPSCs). Climbing fibers of the cerebellum also form synapses with NG2 glia, although producing much larger EPSCs. We aimed to identify mechanisms generating these regional differences in the efficacy of neuron\u2013glia synapses. Combined patch\u2010clamp and RT\u2010PCR analyses allowed for determining structural and functional differences of AMPARs expressed by the glial cells. Comparing pharmacological and molecular data in both regions revealed stronger expression of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    permeable AMPARs in cerebellar NG2 glia. Different expression patterns were found both for AMPAR subunits and their auxiliary proteins. Moreover, experiments using the low\u2010affinity AMPAR antagonist \u03b3\u2010DGG pointed towards higher synaptic glutamate concentrations at cerebellar synapses, likely due to multivesicular release, which contributed to enhanced synaptic efficacy. Finally, we examined short\u2010term plasticity and showed that pre\u2010 and postsynaptic mechanisms contributed to paired\u2010pulse depression at climbing fiber\u2013NG2 glia synapses. Together, our data provide new insights into the molecular and functional specialization of NG2 glia and improve our understanding of the mechanisms underlying neuron\u2013glia synaptic signaling, by highlighting how region\u2010specific differences in AMPAR composition and presynaptic release properties shape this communication in the central nervous system.\n                  <\/jats:p>","DOI":"10.1002\/glia.70107","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T04:26:38Z","timestamp":1764735998000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>AMPA<\/scp>\n                    Receptors in\n                    <scp>NG2<\/scp>\n                    Glia Differently Affect Signal Transduction in the Hippocampus and Cerebellum"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0755-4476","authenticated-orcid":false,"given":"Dario","family":"Tascio","sequence":"first","affiliation":[{"name":"Institute of Cellular Neurosciences I, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nehal","family":"Gebril","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"Jabs","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Henneberger","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, Medical Faculty University of Bonn  Bonn 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