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However, homeostatic microglia dynamically change into disease\u2010associated microglia in human neurodegenerative diseases. Furthermore, microglia\u2010mediated inflammation is required to initiate neuronal regeneration in zebrafish brain. To understand how functional states of microglia are regulated in response to neuronal degeneration and regeneration, we focused on\n                    <jats:italic>pde6c<\/jats:italic>\n                    mutants, a chronic photoreceptor degeneration zebrafish model. We conducted scRNA\u2010seq analysis on microglia in wild\u2010type sibling and\n                    <jats:italic>pde6c<\/jats:italic>\n                    mutant retinas at the onset of photoreceptor degeneration (5 dpf) and M\u00fcller glia\u2010mediated neuronal regeneration (4 wpf). At 5 dpf, retinal microglia consist of three clusters, which correspond to homeostatic, degeneration\u2010response, and stress\u2010response microglia, respectively. The degeneration\u2010response cluster expands in\n                    <jats:italic>pde6c<\/jats:italic>\n                    mutants and expresses genes for neuroprotection and tissue repair. At 4 wpf, retinal microglia comprise four clusters, two of which are specifically produced in\n                    <jats:italic>pde6c<\/jats:italic>\n                    mutants and approach the photoreceptor layer. Furthermore, another cluster is prominently localized in the retinal stem cell niche and shows a transcriptomic profile similar to that of neurogenic\u2010associated microglia (NAM). Comparison of transcriptomic similarity between 4 wpf and 5 dpf microglial clusters revealed that each 4 wpf microglial cluster inherits characteristics of homeostatic, degeneration\u2010response, and stress\u2010response state of 5 dpf microglia in different combinations. Thus, there is a unique heterogeneity of microglia in the initial stage of M\u00fcller glia\u2010mediated regeneration. Taken together, our findings reveal a dynamic change of retinal microglia during the transition from photoreceptor degeneration to M\u00fcller glia\u2010mediated neuronal regeneration in zebrafish.\n                  <\/jats:p>","DOI":"10.1002\/glia.70158","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:56:29Z","timestamp":1776426989000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Dynamic Change of Microglial States Occurs During the Transition From Photoreceptor Degeneration to Regeneration in Zebrafish\n                    <i>pde6c<\/i>\n                    Mutants"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9292-5201","authenticated-orcid":false,"given":"Darshini","family":"Ravishankar","sequence":"first","affiliation":[{"name":"Developmental Neurobiology Unit Okinawa Institute of Science and Technology Graduate University  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