{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:00:48Z","timestamp":1781236848270,"version":"3.54.1"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002839","name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002839","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GR4403\/11\u20101"],"award-info":[{"award-number":["GR4403\/11\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["2025559333543"],"award-info":[{"award-number":["2025559333543"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Previous functional data showed the presence of Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    channels in M\u00fcller cells, among them Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010dependent Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    channels (CaCC). An important step to understanding the role of CaCC in M\u00fcller cell function is the clarification of their molecular identity. Here, we found protein expression of the CaCC anoctamin\u20101 (Ano1, formerly TMEM16A) in M\u00fcller cells in retinal sagittal sections of mouse and rat using immunohistochemistry and proteomic analysis, as well as in the M\u00fcller cell lines ImM10 (murine) and MIO\u2010M1 (human), accompanied by Western blot. Patch\u2010clamp analysis of both cell lines under K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010free conditions showed the activation of membrane currents through an increase in intracellular free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    using either ionomycin, ATP, or adenosine. These currents were sensitive to several blockers: the broad anoctamin blocker niflumic acid, the CaCC blocker CaCCinh\u2010A01, and the Ano1 blocker T16Ainh\u2010A01. To further explore the function of Ano1 in M\u00fcller cells, we investigated the adenosine\u2010dependent volume regulation in mouse M\u00fcller cells in freshly isolated sagittal sections under hypoosmotic challenge by blockage of K\n                    <jats:sup>+<\/jats:sup>\n                    channels. T16Ainh\u2010A01 partially reversed the beneficial effect of adenosine on M\u00fcller cell volume regulation. We conclude that Ano1 is one of the first functionally validated Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    channels in M\u00fcller cells. Ano1 seems to contribute to the adenosine\u2010driven regulation of ion flow across the M\u00fcller cell membrane, which is crucial for clearing extracellular fluid from the retina.\n                  <\/jats:p>","DOI":"10.1002\/glia.70170","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T03:51:50Z","timestamp":1781236310000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anoctamin\u20101 Is a Calcium\u2010Dependent Chloride Channel (\n                    <scp>CaCC<\/scp>\n                    ) in M\u00fcller Cells Involved in Cell Volume Regulation"],"prefix":"10.1002","volume":"74","author":[{"given":"Alissa","family":"Schaefer","sequence":"first","affiliation":[{"name":"Experimental Ophthalmology, Department of Ophthalmology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t, Berlin Institute of Health, Humboldt\u2010University  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9426-0325","authenticated-orcid":false,"given":"Oliver","family":"Bludau","sequence":"additional","affiliation":[{"name":"Department of Physiological Genomics Biomedical Center, Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andjela","family":"Sekulic","sequence":"additional","affiliation":[{"name":"Experimental Ophthalmology, Department of Ophthalmology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t, Berlin Institute of Health, Humboldt\u2010University  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sena","family":"Altunok","sequence":"additional","affiliation":[{"name":"Experimental Ophthalmology, Department of Ophthalmology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t, Berlin Institute of Health, Humboldt\u2010University  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Mergler","sequence":"additional","affiliation":[{"name":"Experimental Ophthalmology, Department of Ophthalmology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t, Berlin Institute of Health, Humboldt\u2010University  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"von Toerne","sequence":"additional","affiliation":[{"name":"Metabolomics and Proteomics Core, Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie M.","family":"Hauck","sequence":"additional","affiliation":[{"name":"Metabolomics and Proteomics Core, Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Grosche","sequence":"additional","affiliation":[{"name":"Department of Physiological Genomics Biomedical Center, Ludwig\u2010Maximilians\u2010Universit\u00e4t M\u00fcnchen  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-8596","authenticated-orcid":false,"given":"Olaf","family":"Strau\u00df","sequence":"additional","affiliation":[{"name":"Experimental Ophthalmology, Department of Ophthalmology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t, Berlin Institute of Health, Humboldt\u2010University  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Reichhart","sequence":"additional","affiliation":[{"name":"Experimental Ophthalmology, Department of Ophthalmology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t, Berlin Institute of Health, Humboldt\u2010University  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2025.101357"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201010910\u20100"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/chan.28065"},{"key":"e_1_2_10_5_1","first-page":"3316","article-title":"Human Muller Glial Cells: Altered Potassium Channel Activity in Proliferative Vitreoretinopathy","volume":"40","author":"Bringmann A.","year":"1999","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10025"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010011\u20100606\u2010z"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.112.277145"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2019\u20100442OC"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1163518"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010014\u20101897\u20106"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424\u2010013\u20101382\u20101"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.043208"},{"key":"e_1_2_10_14_1","first-page":"3033","article-title":"Epinephrine Stimulates Fluid Absorption Across Bovine Retinal Pigment Epithelium","volume":"32","author":"Edelman J. L.","year":"1991","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741\u20107015\u201012\u201087"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2016.04.007"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.052183"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354082"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2009.01.001"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17\u201020\u201007725.1997"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2014.02.006"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067989"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2785716"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20\u201015\u201005733.2000"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.2493\u201009.2009"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010019\u20100287\u20102"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20108993(01)02607\u20105"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06189"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00332.2010"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.4048\u201005.2006"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1098\u20101136(199903)26:1<47::aid\u2010glia5>3.0.co;2\u20105"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.175109"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1017\/s1740925x0500013x"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6474173"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030543"},{"key":"e_1_2_10_36_1","doi-asserted-by":"crossref","first-page":"5965","DOI":"10.1523\/JNEUROSCI.20-16-05965.2000","article-title":"P2X7 Receptors in Muller Glial Cells From the Human Retina","volume":"20","author":"Pannicke T.","year":"2000","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.10.084"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.04.024"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2011"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22477"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23727"},{"key":"e_1_2_10_42_1","doi-asserted-by":"crossref","first-page":"S239","DOI":"10.1139\/y92-267","article-title":"K+ Ion Regulation in Retina","volume":"70","author":"Reichenbach A.","year":"1992","journal-title":"Canadian Journal of Physiology and Pharmacology"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2021.100912"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05875"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0736\u20105748(02)00004\u20107"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.09.003"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108650"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20280"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0736\u20105748(98)00066\u20105"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10305"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.092353"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20270"},{"key":"e_1_2_10_53_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1002\/glia.20113","article-title":"High\u2010Voltage\u2010Activated Calcium\u2009Channels in M\u00fcller Cells Acutely Isolated From Tiger Salamander Retina","volume":"49","author":"Welch N. C.","year":"2005","journal-title":"Glia"},{"key":"e_1_2_10_54_1","first-page":"1858","article-title":"Involvement of A(1) Adenosine Receptors in Osmotic Volume Regulation of Retinal Glial Cells in Mice","volume":"15","author":"Wurm A.","year":"2009","journal-title":"Molecular Vision"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.2009.06541.x"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2011.06.001"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.2007.04992.x"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2024.04.014"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07313"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.70170","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.70170","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.70170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T03:51:54Z","timestamp":1781236314000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.70170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,11]]},"references-count":58,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/glia.70170"],"URL":"https:\/\/doi.org\/10.1002\/glia.70170","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,11]]},"assertion":[{"value":"2025-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70170"}}