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Yet, compared to neurons, our knowledge of the physiological and pathological signaling mechanisms in astrocytes remains limited. As a major challenge, the ultrathin (~10\u2013100\u2009nm) processes of astrocytes render high\u2010throughput quantitative molecular imaging within well\u2010defined cellular contexts very difficult. Here, we introduce a single\u2010molecule localization microscopy\u2010based methodology that achieves unprecedented resolution of the intricate astrocytic arbor in intact brain circuits. Postnatal tagging of the plasma membrane by electroporation in mice resulted in selective and sparse labeling of hippocampal astrocytes and enabled the complete visualization of individual astrocytes with nanoscale precision by using STochastic Optical Reconstruction Microscopy (STORM). We also developed high\u2010yield and easy\u2010to\u2010implement approaches to segment, measure, analyze, and visualize nanoscale molecular information within astrocytic compartments. As a proof\u2010of\u2010concept, we could readily differentiate between synaptic and astrocytic proteins by using dual\u2010color STORM super\u2010resolution imaging. Moreover, we identified cell\u2010type\u2010specific differences in the distribution of monoacylglycerol lipase (MAGL), an enzyme regulating synaptic plasticity in neurons and coupling endocannabinoid signaling to prostaglandin signaling in astrocytes. Our findings demonstrate the feasibility of nanoscale molecular measurements within ultrathin astrocytic processes. Moreover, the results provide insights into the synapse\u2010independent nanoscale arrangement of the astrocytic MAGL pool that controls neuroinflammatory processes.<\/jats:p>","DOI":"10.1002\/glia.70186","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T12:43:50Z","timestamp":1783082630000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Super\u2010Resolution Approach for Astrocyte\u2010Specific Molecular Imaging Reveals the Nanoscale Distribution of Monoacylglycerol Lipase, the Metabolic Node Between Endocannabinoid and Prostaglandin Signaling"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8592-5015","authenticated-orcid":false,"given":"Mikl\u00f3s","family":"Z\u00f6ldi","sequence":"first","affiliation":[{"name":"Department of Psychological and Brain Sciences Indiana University  Bloomington Indiana United States"},{"name":"Laboratory of 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