{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:58:45Z","timestamp":1790395125467,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01NS113556"],"award-info":[{"award-number":["R01NS113556"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Oligodendrocyte precursor cells (OPCs) have been reported to interact with cerebral microvessels, but the extent and spatiotemporal regulation of this relationship across maturation and hypoperfusion\u2010induced stress remain incompletely defined. Here, we quantified OPC\u2010vessel relationships in the mouse corpus callosum using PDGFR\u2010\u03b1\/OLIG2\/CD31 immunohistochemistry and distance\u2010based spatial analysis, classifying OPCs within 10\u2009\u03bcm of CD31+ vessels as perivascular OPCs. We show that while total OPC density decreases from development to adulthood, the proportion of perivascular OPCs increases in the mature brain. Under cerebral hypoperfusion induced by bilateral common carotid artery stenosis (BCAS), OPC density and the enrichment of perivascular OPCs were increased by Day 7 and remained elevated through Days 14\u201328. Proliferative OPCs (Ki67\u2010positive OPCs) were preferentially observed within the vessel\u2010proximal domain, indicating that the perivascular compartment functions as a stress\u2010responsive proliferative niche. To identify regulators of this response, we focused on A\u2010kinase anchoring protein 12 (AKAP12), a scaffolding protein expressed in vascular cells. In middle\u2010aged\n                    <jats:italic>Akap12<\/jats:italic>\n                    global knockout mice subjected to BCAS, hypoperfusion\u2010induced OPC increase was preserved, but perivascular accumulation was reduced. This defect was accompanied by increased IgG leakage without a corresponding reduction in vascular area density, suggesting that AKAP12 supports the integrity of the perivascular microenvironment required for OPC niche remodeling. Together, these findings reveal the perivascular niche as a hypoperfusion\u2010responsive compartment for OPC activation and suggest that AKAP12\u2010dependent vascular barrier integrity contributes to the maintenance of this oligovascular niche.\n                  <\/jats:p>","DOI":"10.1002\/glia.70222","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:07:42Z","timestamp":1789459662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>AKAP12<\/scp>\n                    Regulates Perivascular\n                    <scp>OPC<\/scp>\n                    Accumulation in the Corpus Callosum During Cerebral Hypoperfusion"],"prefix":"10.1002","volume":"74","author":[{"given":"Shintaro","family":"Kimura","sequence":"first","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"},{"name":"Life Science Research Center Gifu University  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-602X","authenticated-orcid":false,"given":"Munehiro","family":"Demura","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5813-1386","authenticated-orcid":false,"given":"Hajime","family":"Takase","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akane","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"},{"name":"Department of Neurology Mie University Graduate School of Medicine  Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selin","family":"Dogan","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1334-0079","authenticated-orcid":false,"given":"Shivani","family":"Jajoo","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Lok","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School  Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2233-9569","authenticated-orcid":false,"given":"Byung Gon","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of Brain Science and Neurology Ajou University School of Medicine  Suwon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1006-4943","authenticated-orcid":false,"given":"Akihiro","family":"Shindo","sequence":"additional","affiliation":[{"name":"Department of Neurology Mie University Graduate School of Medicine  Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9204-207X","authenticated-orcid":false,"given":"Irwin H.","family":"Gelman","sequence":"additional","affiliation":[{"name":"Department of Cancer Genetics and Genomics Roswell Park Comprehensive Cancer Center  Buffalo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1615-3258","authenticated-orcid":false,"given":"Ken","family":"Arai","sequence":"additional","affiliation":[{"name":"Neuroprotection Research Laboratories, Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical 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