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Here, we identify a pathogenic subpopulation of astrocytes expressing phosphodiesterase 7B (PDE7B) that contributes to BBB disruption. Using a colitis\u2010induced BBB opening model and single\u2010cell RNA sequencing, we found PDE7B\n                    <jats:sup>high<\/jats:sup>\n                    astrocytes enriched at sites of BBB breakdown. In both human demyelinated lesions and the experimental autoimmune encephalomyelitis mouse model, elevated astrocytic PDE7B expression correlated with vascular leakage. In\u00a0vivo and in\u00a0vitro experiments confirmed that astrocytic PDE7B impairs BBB integrity, in part via effects on endothelial cells. Mechanistically, elevated PDE7B promotes BBB disruption through astrocyte\u2010derived extracellular vesicles enriched in miR\u2010155\u20105p, which disrupts endothelial tight junction integrity and focal adhesion\u2010associated signaling. These findings identify astrocytic PDE7B as a regulatory and therapeutic target of neurovascular integrity and provide new insight into how glial signaling contributes to BBB dysfunction in demyelinating diseases.\n                  <\/jats:p>","DOI":"10.1002\/glia.70223","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:15:36Z","timestamp":1788876936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Astrocytic\n                    <scp>PDE7B<\/scp>\n                    Drives Blood\u2013Brain Barrier Breakdown via Extracellular Vesicles in Demyelinating Disease"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3715-296X","authenticated-orcid":false,"given":"Jing","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou Zhejiang 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