{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:49:54Z","timestamp":1792039794324,"version":"4.4.0"},"reference-count":64,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HI1414\/9\u20101"],"award-info":[{"award-number":["HI1414\/9\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["TMCG\u20103_223118"],"award-info":[{"award-number":["TMCG\u20103_223118"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Myelin is a highly complex membranous structure wrapped around axons by oligodendrocytes or Schwann cells in the central and peripheral nervous system, respectively. Fluorescent labeling is widely used to study the structure and dynamics of myelin. Combining structural with functional imaging requires labeling of myelin with red fluorescence, as many functional sensors, including Ca\n                    <jats:sup>2+<\/jats:sup>\n                    indicators and genetically encoded metabolite sensors, fluoresce in the green spectral range. However, in\u00a0vivo tools enabling red fluorescent labeling of myelinating cells and their myelin sheaths remain limited. Here, we generated a set of seven transgenic mouse lines expressing a membrane\u2010targeted variant of the red fluorescent protein tdTomato in myelinating oligodendrocytes and Schwann cells throughout the nervous system. The mouse lines provide a variety of expression patterns ranging from wide\u2010spread labeling of myelin to a rather sparse expression, the latter enabling visualization of individual oligodendrocytes and their associated myelin sheaths. In the peripheral nervous system, the pattern of fluorescence in sciatic nerves indicates predominant localization of tdTomato to non\u2010compact myelin compartments including the inner and outer tongues, paranodal loops and Schmidt\u2010Lanterman incisures. In summary, our work provides a set of novel mouse lines with myelin labeled by red fluorescence, which are compatible with diverse imaging modalities in the green spectral range enabling integrated structural and functional imaging.\n                  <\/jats:p>","DOI":"10.1002\/glia.70246","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:42:20Z","timestamp":1792039340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Red Fluorescent Labeling of Myelin by Membrane\u2010Targeted\n                    <scp>tdTomato<\/scp>\n                    in Transgenic Mouse Lines"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3368-0028","authenticated-orcid":false,"given":"Anja","family":"Reinert","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Carl Ludwig Institute for Physiology Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7224-8607","authenticated-orcid":false,"given":"Ulrike","family":"Winkler","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Carl Ludwig Institute for Physiology Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8343-9972","authenticated-orcid":false,"given":"Sandra","family":"Goebbels","sequence":"additional","affiliation":[{"name":"Department of Neurogenetics Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2062-457X","authenticated-orcid":false,"given":"Lina","family":"Komarek","sequence":"additional","affiliation":[{"name":"Department of Neurogenetics Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2902-7165","authenticated-orcid":false,"given":"Wiebke","family":"M\u00f6bius","sequence":"additional","affiliation":[{"name":"Department of Neurogenetics Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Electron Microscopy City Campus Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3820-1214","authenticated-orcid":false,"given":"Henri Seraf","family":"Zanker","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology University of Zurich  Zurich Switzerland"},{"name":"Neuroscience Center Zurich University and ETH Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-9773","authenticated-orcid":false,"given":"Karl","family":"Schoknecht","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Carl Ludwig Institute for Physiology Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4871-2686","authenticated-orcid":false,"given":"Grit","family":"Bornschein","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Carl Ludwig Institute for Physiology Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9516-423X","authenticated-orcid":false,"given":"Hartmut","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Carl Ludwig Institute for Physiology Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5323-7958","authenticated-orcid":false,"given":"Robert","family":"Fledrich","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Anatomy Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-1652","authenticated-orcid":false,"given":"Ruth M.","family":"Stassart","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Paul Flechsig Institute, Centre for Neuropathology and Brain Research Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra G.","family":"Hirrlinger","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Medical\u2010Experimental Center Leipzig University  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8724-9666","authenticated-orcid":false,"given":"Klaus\u2010Armin","family":"Nave","sequence":"additional","affiliation":[{"name":"Department of Neurogenetics Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7710-5738","authenticated-orcid":false,"given":"Hauke B.","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Neurogenetics Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Neurochemistry Group Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"},{"name":"Faculty for Biology and Psychology University of G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3886-8369","authenticated-orcid":false,"given":"Aiman S.","family":"Saab","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology University of Zurich  Zurich Switzerland"},{"name":"Neuroscience Center Zurich University and ETH Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6327-0089","authenticated-orcid":false,"given":"Johannes","family":"Hirrlinger","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Carl Ludwig Institute for Physiology Leipzig University  Leipzig Germany"},{"name":"Department of Neurogenetics Max Planck Institute for Multidisciplinary Sciences  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.08.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264\u2010013\u20101322\u20102"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.071001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adr4661"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050001"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.201376"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1516"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.01.016"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102818.2006.01706.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010025\u201001973\u20108"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010026\u201010312\u20101"},{"issue":"9","key":"e_1_2_11_13_1","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1038\/s41593-026-02383-0","article-title":"An Activity\u2010Modulated Transport Route Across Myelin (TRAM) for Motor\u2010Driven Organelle Transfer in Oligodendrocytes","volume":"29","author":"Chapple K. J.","year":"2026","journal-title":"Nature Neuroscience"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1113540109"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.11.039"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2024.1408182"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22751"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006120\u20106"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2025.11.009"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13577"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201018172\u20107"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010018\u20100236\u2010y"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0896\u20106273(00)00084\u20102"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02033"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(89)90054\u20108"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460\u20109568.2004.03689.x"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2005.08.011"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3390"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010018\u20100121\u20105"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904764106"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44303\u2010025\u201000098\u20108"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2020.02.011"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3263"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00377"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010023\u201001558\u20103"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3078"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22\u201003\u201000876.2002"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf6463"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3991"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.03.031"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010neuro\u2010100120\u2010122621"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110647"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13967"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041360"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3048\u201013.2013"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.02.012"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3495"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2412"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010023\u201001568\u2010z"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5460\u201013.2014"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24614"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041359"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.11.044"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201018632\u20100"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.12.053"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000001370"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1939.127.2.211"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201059400\u20102"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000943"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.24241"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20109072\u20106_20"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010020\u201000432\u20101"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP283832"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255\u2010020\u20100170\u20104"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.70246","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.70246","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.70246","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:42:29Z","timestamp":1792039349000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.70246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":64,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["10.1002\/glia.70246"],"URL":"https:\/\/doi.org\/10.1002\/glia.70246","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"2026-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70246"}}