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To identify regulatory targets that promote retinal inflammation, we established an experimental autoimmune uveoretinitis (EAU) model by immunizing C57BL\/6J mice with IRBP\n                    <jats:sub>651\u2013670<\/jats:sub>\n                    and dynamically evaluated retinal inflammation after induction; fundus lesions were prominent, with the first 14\u2009days representing the initiation phase, Days 14\u201328 the amplification phase, Days 28\u201335 the peak phase, and Days 35\u201342 the regression phase. We characterized retinal cellular alterations from the pre\u2010inflammatory stage to the peak stage using single\u2010cell transcriptomic analysis, and found that retinal immune cells changed in parallel with EAU severity, while immune\u2010cell interactions were strongest on Day 14, especially between microglia and T cells through multiple chemokine\u2010related pathways. We then identified key cell populations and candidate targets through integrated analyses and functional validation, and\n                    <jats:italic>Ifi27l2a<\/jats:italic>\n                    emerged as a core gene associated with inflammatory and immune responses in retinal microglia on Day 14. In\u00a0vitro knockdown of\n                    <jats:italic>Ifi27l2a<\/jats:italic>\n                    in LPS\u2010activated BV\u20102 cells reduced inflammatory mediator production and abolished Th1\/Th17 induction. In\u00a0vivo targeting of\n                    <jats:italic>Ifi27l2a<\/jats:italic>\n                    attenuated EAU severity and restored the balance of T\u2010cell subsets. These findings indicate that microglia are highly activated before retinal inflammation becomes prominent and that\n                    <jats:italic>Ifi27l2a<\/jats:italic>\n                    regulates this early pathogenic process.\n                  <\/jats:p>","DOI":"10.1002\/glia.70247","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T05:32:29Z","timestamp":1792474349000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Ifi27l2a<\/i>\n                    Facilitates the Recruitment of Pathogenic T Cells by Retinal Microglia in the Initiation Phase of Experimental Autoimmune Uveoretinitis"],"prefix":"10.1002","volume":"74","author":[{"given":"Wenna","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Henan International Joint Research Laboratory for Ocular Immunology and Retinal Injury Repair, Henan Province Eye Hospital, The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pucheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Henan International Joint Research Laboratory for Ocular Immunology and Retinal Injury Repair, Henan Province Eye Hospital, The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunpeng","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Henan International Joint Research Laboratory for Ocular Immunology and Retinal Injury Repair, Henan Province Eye Hospital, The First Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Henan International Joint Research 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