{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T03:31:12Z","timestamp":1771039872390,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T00:00:00Z","timestamp":1087948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background.<\/jats:title><jats:p>The purpose of this study was to investigate the possibility of identifying laryngopharyngeal cancers by nuclear chromatin texture feature analysis of cell nuclei from mucosal scrapings obtained from clinically and cytologically noncancerous areas of the soft palate in patients with cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>The collective consisted of 68 controls and 77 cases of laryngopharyngeal carcinomas. After Feulgen staining, 3000 cell nuclei were automatically measured using a high\u2010resolution image analyser (CytoSavant Oncometrics, Vancouver, BC, Canada). Texture features were extracted for calculation of a discriminant function, which allows the two groups to be distinguished.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>Two parameters allowed the two populations to be distinguished. The classifier reached an overall performance of 72.7% sensitivity, 82.4% specificity, a positive predictive value of 80.5%, a negative predictive value of 75.1%, and an area under the receiver operating characteristics (ROC) curve of 0.7754.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion.<\/jats:title><jats:p>Our work shows that subtle changes in the chromatin distribution in cell nuclei from ostensibly normal cells in the vicinity of carcinomas are demonstrable in the oral cavity of patients suffering from laryngopharyngeal cancers. It may be possible to develop this method into a valuable clinical tool to reduce the high rate of delayed diagnosis of oral and laryngopharyngeal cancers. \u00a9 2004 Wiley Periodicals, Inc. <jats:italic>Head Neck<\/jats:italic> <jats:bold>26:<\/jats:bold> 694\u2013700, 2004<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.20020","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T23:01:41Z","timestamp":1088031701000},"page":"694-700","source":"Crossref","is-referenced-by-count":7,"title":["High\u2010resolution image cytometry on smears of normal oral mucosa: a possible approach for the early detection of laryngopharyngeal cancers"],"prefix":"10.1002","volume":"26","author":[{"given":"Andreas","family":"Neher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnter","family":"\u00d6fner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Appenroth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Gschwendtner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,6,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19890815)64:4<932::AID-CNCR2820640428>3.0.CO;2-Y"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890230029007"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10356"},{"key":"e_1_2_7_5_2","first-page":"137","article-title":"Systemic cellular changes in material from human and animal tissues in presence of tumors","author":"Nieburgs HE","year":"1959","journal-title":"Trans Annu Mtg Inter\u2010Soc Cytol Counc, 7th"},{"key":"e_1_2_7_6_2","first-page":"80","article-title":"Buccal cell changes in patients with malignant tumors","volume":"11","author":"Nieburgs HE","year":"1962","journal-title":"Lab Invest"},{"key":"e_1_2_7_7_2","first-page":"445","article-title":"Recent progress in the interpretation of malignancy\u2010associated changes (MAC)","volume":"12","author":"Nieburgs HE","year":"1968","journal-title":"Acta Cytol"},{"key":"e_1_2_7_8_2","first-page":"262","article-title":"Cellular changes in liver tissue adjacent and distant to malignant tumors","volume":"80","author":"Nieburgs HE","year":"1965","journal-title":"Arch Pathol"},{"key":"e_1_2_7_9_2","first-page":"415","article-title":"Malignancy\u2010associated changes (MAC) in blood and bone marrow cells of patients with malignant tumors","volume":"11","author":"Nieburgs HE","year":"1967","journal-title":"Acta Cytol"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(196304)16:4<480::AID-CNCR2820160409>3.0.CO;2-Y"},{"key":"e_1_2_7_11_2","first-page":"23","article-title":"Chromatin appearance in intermediate cells from patients with uterine cancer","volume":"25","author":"Bibbo M","year":"1981","journal-title":"Acta Cytol"},{"key":"e_1_2_7_12_2","first-page":"29","article-title":"Diagnostic marker displays for intermediate cells from the uterine cervix","volume":"26","author":"Bartels PH","year":"1982","journal-title":"Acta Cytol"},{"key":"e_1_2_7_13_2","first-page":"154","article-title":"Chromatin measurements in Feulgen stained intermediate cells from normal and abnormal cervical smears","volume":"4","author":"Vooijs PG","year":"1982","journal-title":"Anal Quant Cytol"},{"key":"e_1_2_7_14_2","first-page":"157","article-title":"Diagnostic relevance of visually non\u2010suspect cells for cancer diagnosis","volume":"4","author":"Reinhardt ER","year":"1982","journal-title":"Anal Quant Cytol"},{"key":"e_1_2_7_15_2","first-page":"243","article-title":"Intermediate cell markers for malignancy. 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