{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:48:12Z","timestamp":1791114492622,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,7,26]],"date-time":"2007-07-26T00:00:00Z","timestamp":1185408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Human papillomavirus 16 (HPV16) has been associated with head and neck squamous cell carcinoma (HNSCC) in up to 60% of sampled specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To understand better the viral genes required to transform human tonsil epithelial cells (HTEC), we isolated HTEC's and transduced them with retroviral vectors containing HPV16<jats:italic>E6<\/jats:italic>and<jats:italic>E7<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immortalization and anchorage\u2010independent growth of HTEC's only occurred with expression of<jats:italic>E6<\/jats:italic>and<jats:italic>E7<\/jats:italic>with resultant degradation of p53. However, cells expressing<jats:italic>E6<\/jats:italic>lacking the PSD\u201095\/disc\u2010large\/Zo\u20101 (PDZ) motif did not immortalize or grow anchorage independent. Telomerase activity and degradation of p53 were similar for wild\u2010type and mutant E6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The mechanism of oncogenic transformation by E6 in HTEC's is dependent on the PDZ binding motif. Identification of pathways affected by the interaction of E6 and PDZ domain containing proteins will further our understanding of how HPV causes HNSCC and will provide potential therapeutic targets. \u00a9 2007 Wiley Periodicals, Inc. Head Neck, 2008<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.20673","type":"journal-article","created":{"date-parts":[[2007,7,26]],"date-time":"2007-07-26T18:16:39Z","timestamp":1185473799000},"page":"139-147","source":"Crossref","is-referenced-by-count":44,"title":["Deletion of the PDZ motif of HPV16 E6 preventing immortalization and anchorage\u2010independent growth in human tonsil epithelial cells"],"prefix":"10.1002","volume":"30","author":[{"given":"William C.","family":"Spanos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Geiger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George F.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron 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