{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T19:40:23Z","timestamp":1697485223742},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,2,26]],"date-time":"2008-02-26T00:00:00Z","timestamp":1203984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The relative expression level of trefoil factor 3 (<jats:italic>TFF3<\/jats:italic>) mRNA to galectin\u20103 <jats:italic>(LGALS3<\/jats:italic>) mRNA (T\/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T\/G ratio in aspirates is difficult.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber\u2010EP4 antibody and the other was filtration through a nylon mesh.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After selection with Ber\u2010EP4 antibody, T\/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T\/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mesh filtration of aspirates proved superior results for the measurement of T\/G ratio. \u00a9 2008 Wiley Periodicals, Inc. 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