{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:27:09Z","timestamp":1783488429681,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T00:00:00Z","timestamp":1248220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background.<\/jats:title><jats:p>An activating point mutation of the <jats:italic>BRAF<\/jats:italic> oncogene results in a V600E amino acid missense mutation found in a majority of papillary thyroid carcinomas (PTC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods.<\/jats:title><jats:p>In this study, 28 matched tumor and serum samples obtained from patients with both benign and malignant thyroid disorders were analyzed for <jats:italic>BRAF<\/jats:italic> mutation using a gap\u2010ligase chain reaction technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results.<\/jats:title><jats:p>The <jats:italic>BRAF<\/jats:italic> mutation was absent in tumor DNA samples obtained from patients with benign adenomas, follicular neoplasms or carcinoma, and thyroid lymphoma. In contrast, 5 of 14 PTC tumors were positive for the <jats:italic>BRAF<\/jats:italic> mutation. Moreover, 3 of 14 patients with PTC were positive for <jats:italic>BRAF<\/jats:italic> mutation in serum and tumor. Of these 3 patients, 2 had lymph node metastasis and 2 had PTC in background of the Hashimoto's thyroiditis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions.<\/jats:title><jats:p>The detection of free circulating mutant <jats:italic>BRAF<\/jats:italic> in patients with PTC is possible and future studies are warranted to determine its clinical significance. \u00a9 2009 Wiley Periodicals, Inc. Head Neck, 2010<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.21178","type":"journal-article","created":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T15:50:36Z","timestamp":1248277836000},"page":"229-234","source":"Crossref","is-referenced-by-count":38,"title":["Detectable <i>BRAF<\/i> mutation in serum DNA samples from patients with papillary thyroid carcinomas"],"prefix":"10.1002","volume":"32","author":[{"given":"Tony C. Y.","family":"Chuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Y. 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