{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T06:39:47Z","timestamp":1719988787898},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,9,10]],"date-time":"2012-09-10T00:00:00Z","timestamp":1347235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The purpose of this study was to compare the major thrombin receptor protease\u2010activated receptor\u20101 (PAR\u20101) expression in normal human buccal mucosa and oral submucous fibrosis (OSF) specimens and further explore the potential mechanisms that may lead to induce PAR\u20101 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty OSF and 10 normal buccal mucosa specimens were examined by immunohistochemistry. Buccal mucosal fibroblasts (BMFs) were challenged with arecoline by using Western blot analysis. <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:italic>L<\/jats:italic>\u2010cysteine (NAC), LY294002, herbimycin A, NS\u2010398, and PD98059 were added to find the possible regulatory mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PAR\u20101 expression was significantly higher in OSF specimens (<jats:italic>p<\/jats:italic> &lt; .05). Arecoline was found to elevate PAR\u20101 expression in a dose\u2010dependent and time\u2010dependent manner (<jats:italic>p<\/jats:italic> &lt; .05). The addition of NAC, LY294002, herbimycin A, NS398, and PD98059 markedly inhibited the arecoline\u2010induced PAR\u20101 expression (<jats:italic>p<\/jats:italic> &lt; .05).<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:italic>Conclusion<\/jats:italic><\/jats:title><jats:p>PAR\u20101 expression is significantly upregulated in areca quid chewing\u2010associated OSF. Arecoline\u2010induced PAR\u20101 expression was downregulated by NAC, LY294002, herbimycin A, NS398, and PD98059. \u00a9 2012 Wiley Periodicals, Inc. Head Neck, 2013<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.23130","type":"journal-article","created":{"date-parts":[[2012,9,10]],"date-time":"2012-09-10T12:55:59Z","timestamp":1347281759000},"page":"1314-1318","source":"Crossref","is-referenced-by-count":7,"title":["Regulation of protease\u2010activated receptor\u20101 expression in human buccal fibroblasts stimulated with arecoline"],"prefix":"10.1002","volume":"35","author":[{"given":"Chung\u2010Hung","family":"Tsai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiuan\u2010Shinn","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu\u2010Mei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Chao","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,9,10]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.08.005"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10006-010-0219-8"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1994.tb00258.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/13556210120091482"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1998.tb02096.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00083-5"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2003.00004.x"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00045-8"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(02)00123-9"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01272.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2004.01041.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00288.x"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.05.006"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.06.007"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.08.009"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2007.00633.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2009.01523.x"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35025229"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.36.23290"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62354-1"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510270221"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-005-0078-0"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00444.2007"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.3.455"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst0300194"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2009.04.004"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(00)00262-9"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.296.5573.1655"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/35077225"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2010.00998.x"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:31:2:151"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2007.11.004"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhed.23130","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.23130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T16:11:54Z","timestamp":1700669514000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.23130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,10]]},"references-count":32,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1002\/hed.23130"],"URL":"https:\/\/doi.org\/10.1002\/hed.23130","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,10]]}}}