{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:29:41Z","timestamp":1783484981031,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,3,6]],"date-time":"2013-03-06T00:00:00Z","timestamp":1362528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Multiple endocrine neoplasia type 2B (MEN2B) is the rarest and most aggressive form of MEN2. MEN2B cases usually carry either an M918T or A883T mutation of the <jats:italic>RET<\/jats:italic>, but to date, there are 3 atypical MEN2B caused by tandem mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>A 32\u2010year\u2010old woman with no family history of medullary thyroid carcinoma (MTC) presented with a neck tumor and multiple mucosal nodules. She was diagnosed with MEN2B. Genetic analyses of <jats:italic>RET<\/jats:italic> revealed that she had 2 mutations, Q781R and V804M. Subclone and genetic analyses revealed that Q781R was on the paternal allele and V804M was a <jats:italic>de novo<\/jats:italic>. <jats:italic>In silico<\/jats:italic> analysis of the tandem mutations showed a high prediction score.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We describe a novel combination of tandem <jats:italic>RET<\/jats:italic> mutations (Q781R\/V804M) in a MEN2B\u2010like patient. <jats:italic>In silico<\/jats:italic> analysis showed a high prediction score, which was compatible with the clinical phenotype in the present case. \u00a9 2012 Wiley Periodicals, Inc. Head Neck 35: E363\u2013E368, 2013<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.23241","type":"journal-article","created":{"date-parts":[[2013,3,6]],"date-time":"2013-03-06T17:17:34Z","timestamp":1362590254000},"source":"Crossref","is-referenced-by-count":29,"title":["Novel tandem germline <i>RET<\/i> proto\u2010oncogene mutations in a patient with multiple endocrine neoplasia type 2B: Report of a case and a literature review of tandem <i>RET<\/i> mutations with <i>in silico<\/i> analysis"],"prefix":"10.1002","volume":"35","author":[{"given":"Kanako\u2013Tanase","family":"Nakao","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism National Hospital Organization, Kyoto Medical Center Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Usui","sequence":"additional","affiliation":[{"name":"Clinical Research Institute National Hospital Organization, Kyoto Medical Center Kyoto 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