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To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2\u2010photon microscopy were used to evaluate tumor progress and spread.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology. \u00a9 2014 Wiley Periodicals, Inc. <jats:italic>Head Neck<\/jats:italic> 36: 1638\u20131647, 2014<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.23500","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:20:10Z","timestamp":1381540810000},"page":"1638-1647","source":"Crossref","is-referenced-by-count":7,"title":["Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer"],"prefix":"10.1002","volume":"36","author":[{"given":"Peter","family":"Szaniszlo","sequence":"first","affiliation":[{"name":"Department of Otolaryngology UTMB Health Cancer Center Galveston Texas"},{"name":"UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Fennewald","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology UTMB Health Cancer Center Galveston Texas"},{"name":"UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suimin","family":"Qiu","sequence":"additional","affiliation":[{"name":"UTMB Health Cancer Center Galveston Texas"},{"name":"Department of Pathology UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Kantara","sequence":"additional","affiliation":[{"name":"UTMB Health Cancer Center Galveston Texas"},{"name":"Department of Neuroscience and Cell Biology UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuya","family":"Shilagard","sequence":"additional","affiliation":[{"name":"Center for Biomedical Engineering UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gracie","family":"Vargas","sequence":"additional","affiliation":[{"name":"Center for Biomedical Engineering UTMB Health Cancer Center Galveston Texas"},{"name":"Department of Neuroscience and Cell Biology UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicente A.","family":"Resto","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology UTMB Health Cancer Center Galveston Texas"},{"name":"UTMB Health Cancer Center Galveston Texas"},{"name":"Department of Biochemistry and Molecular Biology UTMB Health Cancer Center Galveston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/982879"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2006.01.062"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20138"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20607"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-003-0581-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-437"},{"key":"e_1_2_6_9_1","first-page":"211","article-title":"Clinicopathological predictors of lymphatic metastasis in HNSCC: implications for molecular mechanisms of metastatic disease","volume":"8","author":"Goldson TM","year":"2010","journal-title":"J Exp Ther Oncol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000161338.54515.b1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2010.01.026"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.20222"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-009-0955-2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdi452"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-3284-1-32"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.20397"},{"key":"e_1_2_6_17_1","first-page":"713","article-title":"Heterotransplantation of human epidermoid carcinomas to the mouse mutant nude","volume":"80","author":"Povlsen CO","year":"1972","journal-title":"Acta Pathol Microbiol Scand A"},{"key":"e_1_2_6_18_1","first-page":"159","article-title":"Heterotransplantation of human adenocarcinomas of the colon and rectum to the mouse mutant nude. 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