{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:51:17Z","timestamp":1776401477777,"version":"3.51.2"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T00:00:00Z","timestamp":1396483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council, Taipei, Taiwan","doi-asserted-by":"crossref","award":["NSC-1012314B002096MY3"],"award-info":[{"award-number":["NSC-1012314B002096MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early growth response\u20101 (Egr\u20101) protein plays an important role in many human fibrotic diseases. Areca nut chewing is the most important risk factor of oral submucous fibrosis (OSF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Egr\u20101 protein expression in OSF was examined using antibody to Egr\u20101. Arecoline\u2010induced Egr\u20101 expression and its signaling pathways were assessed by Western blot analyses in human buccal mucosal fibroblasts (BMFs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Elevated Egr\u20101 staining was observed in epithelial cells, fibroblast, and inflammatory cells in 7 of 10 OSF cases. Arecoline, a main alkaloid found in the areca nut, stimulated Egr\u20101 synthesis in BMFs. Pretreatment with antioxidant N\u2010acetyl\u2010L\u2010cysteine, c\u2010Jun NH2\u2010terminal kinase inhibitor SP600125, and extracellular signal\u2010regulated kinase inhibitor PD98059 significantly reduced arecoline\u2010induced Egr\u20101 synthesis. Epigallocatechin\u20103\u2010gallate (EGCG) inhibited arecoline\u2010induced Egr\u20101 synthesis and collagen gel contraction in a dose\u2010responsive manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Constitutive Egr\u20101 expression during areca nut chewing may play a role in the pathogenesis of OSF. EGCG could be a good candidate for prevention or treatment of OSF. \u00a9 2014 Wiley Periodicals, Inc. <jats:italic>Head Neck<\/jats:italic> <jats:bold>37<\/jats:bold>: 493\u2013497, 2015<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.23614","type":"journal-article","created":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T08:11:48Z","timestamp":1389946308000},"page":"493-497","source":"Crossref","is-referenced-by-count":21,"title":["Arecoline stimulated early growth response\u20101 production in human buccal fibroblasts: Suppression by epigallocatechin\u20103\u2010gallate"],"prefix":"10.1002","volume":"37","author":[{"given":"Yu\u2010Ping","family":"Hsieh","sequence":"first","affiliation":[{"name":"School of Dentistry National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Dentistry National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny Zwei\u2010Chieng","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Dentistry National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Pin","family":"Chiang","sequence":"additional","affiliation":[{"name":"School of Dentistry National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Ting","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Dentistry National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital Hsin\u2010Chu Branch Hsin\u2010Chu Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark Yen\u2010Ping","family":"Kuo","sequence":"additional","affiliation":[{"name":"School of Dentistry National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2005.00325.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.08.005"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10006-010-0219-8"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(01)00045-8"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(02)00123-9"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2009.04.004"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2786"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2011.03.005"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4131"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603270200"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.080382"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21863"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-012-0713-5"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513488139"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055122"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.090241"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0182"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068943"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.155143"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.931806"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(03)00048-2"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2001"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21104"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22289"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003421"},{"key":"e_1_2_5_27_1","first-page":"226","article-title":"Early growth response 1\u2014a transcription factor in the crossfire of signal transduction cascades","volume":"48","author":"Pagel JI","year":"2011","journal-title":"Indian J Biochem Biophys"}],"container-title":["Head &amp; 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