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Despite this, no HPV16\u2010positive tonsillar cancer cell line has been established in nude mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB\/c, nu\/nu).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4\u20132.9) of HPV16\/cell and displayed 99.9% (7904\/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2\u2010binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in 14 xenografts from the second passage. From this passage, a viral load of 6.4 copies\/cell (range = 4.6\u20139.6) and 3.7 (range = 1.0\u20135.5) E7\u2010mRNA transcripts\/HPV16\u2010genome were detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This xenograft represents the first established HPV16\u2010positive tonsillar tumor in nude mice and could provide an experimental system of HPV16\u2010positive tonsillar cancers. \u00a9 2015 Wiley Periodicals, Inc. <jats:italic>Head Neck<\/jats:italic> 38: 417\u2013425, 2016<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.23918","type":"journal-article","created":{"date-parts":[[2014,10,28]],"date-time":"2014-10-28T16:34:33Z","timestamp":1414514073000},"page":"417-425","source":"Crossref","is-referenced-by-count":10,"title":["Establishment and characterization of a human papillomavirus type 16\u2013positive tonsillar carcinoma xenograft in BALB\/c nude mice"],"prefix":"10.1002","volume":"38","author":[{"given":"Boitelo 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