{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T21:40:35Z","timestamp":1753738835922,"version":"3.37.3"},"reference-count":22,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T00:00:00Z","timestamp":1435276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council Taiwan","doi-asserted-by":"publisher","award":["102-2314-B-002-106"],"award-info":[{"award-number":["102-2314-B-002-106"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Artificial salivary gland replacement would be an ideal treatment for xerostomia. In vivo, salivary gland cells are surrounded by a complex stromal environment in which fibroblasts are the main cell type in proximity to the gland cells. However, very little is known about the relationship between these fibroblasts and the gland cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Parotid gland acinar cells (PGACs) and fibroblasts from the same human gland were cocultured. PGAC function\u2010related protein expression was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of \u03b1\u2010amylase in PGACs was increased in a fibroblast ratio\u2010dependent manner. Both fibroblast\u2010conditioned medium and direct coculture also significantly enhanced the PGAC expression of \u03b1\u2010amylase. Basic fibroblast growth factor (bFGF) seems to be a regulator of \u03b1\u2010amylase expression in PGACs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>An appropriate number of fibroblasts in contact with the PGACs is necessary to promote PGAC function. Fibroblast\u2010secreted bFGF may play a paracrine signaling role in the regulation of \u03b1\u2010amylase expression in PGACs. \u00a9 2015 Wiley Periodicals, Inc.<jats:italic>Head Neck<\/jats:italic><jats:bold>38<\/jats:bold>: E279\u2013E286, 2016<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.23986","type":"journal-article","created":{"date-parts":[[2014,12,24]],"date-time":"2014-12-24T09:47:48Z","timestamp":1419414468000},"source":"Crossref","is-referenced-by-count":8,"title":["Effects of fibroblasts on the function of acinar cells from the same human parotid gland"],"prefix":"10.1002","volume":"38","author":[{"given":"Ya\u2010Shuan","family":"Chou","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine and College of Engineering, National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Chong","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine and College of Engineering, National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai\u2010Horng","family":"Young","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering College of Medicine and College of Engineering, National Taiwan University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Jen","family":"Lou","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National Taiwan University Hospital and College of Medicine Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.1989.0211"},{"issue":"3","key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1288\/00005537-199403000-00016","article-title":"Collagen gel cultures of normal salivary gland: conditions for continued proliferation and maintenance of major cell phenotypes in vitro","volume":"104","author":"Burford\u2013Mason AP","year":"1994","journal-title":"Laryngoscope"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041550202"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2006.00138.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000154726.77915.cc"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2006.0054"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.172"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.060"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2010.0214"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2011.12.004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.10228"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/10763270152044206"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.3.1127"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0251"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2008.0044"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21465"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22664"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016480260092372"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.07914.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2002-0109"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199812)177:4<628::AID-JCP13>3.0.CO;2-L"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2004.10.995"}],"container-title":["Head &amp; 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