{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:49:19Z","timestamp":1790192959709,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,9,27]],"date-time":"2017-09-27T00:00:00Z","timestamp":1506470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"iStat Biomedical Co, Ltd"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Effective biomarkers for oral cancer screening are important for early diagnosis and treatment of oral cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Oral epithelial cell samples collected by mouth rinse were obtained from 65 normal control subjects, 108 patients with oral potentially malignant disorders, and 94 patients with oral squamous cell carcinoma (OSCC). Methylation levels of zinc\u2010finger protein 582 (<jats:italic>ZNF582<\/jats:italic>) and paired\u2010box 1 (<jats:italic>PAX1<\/jats:italic>) genes were quantified by real\u2010time methylation\u2010specific polymerase chain reaction after bisulfite conversion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An abrupt increase in methylated <jats:italic>ZNF582<\/jats:italic> (<jats:italic>ZNF582<jats:sup>m<\/jats:sup><\/jats:italic>) and <jats:italic>PAX1<\/jats:italic> (<jats:italic>PAX1<jats:sup>m<\/jats:sup><\/jats:italic>) levels and positive rates from mild dysplasia to moderate\/severe dysplasia, indicating that both <jats:italic>ZNF582<jats:sup>m<\/jats:sup><\/jats:italic> and <jats:italic>PAX1<jats:sup>m<\/jats:sup><\/jats:italic> are effective biomarkers for differentiating moderate dysplasia or worse (MODY+) oral lesions. When <jats:italic>ZNF582<jats:sup>m<\/jats:sup>\/PAX1<jats:sup>m<\/jats:sup><\/jats:italic> tests were used for identifying MODY+ oral lesions, the sensitivity, specificity, and odds ratio (OR) were 0.65\/0.64, 0.75\/0.82, and 5.6\/8.0, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hypermethylated <jats:italic>ZNF582<\/jats:italic> and <jats:italic>PAX1<\/jats:italic> genes in oral epithelial cells collected by mouth rinse are effective biomarkers for the detection of oral dysplasia and oral cancer.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.24958","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T15:43:49Z","timestamp":1506613429000},"page":"355-368","source":"Crossref","is-referenced-by-count":47,"title":["Hypermethylated <i>ZNF582<\/i> and <i>PAX1<\/i> genes in mouth rinse samples as biomarkers for oral dysplasia and oral cancer detection"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4674-4892","authenticated-orcid":false,"given":"Shih\u2010Jung","family":"Cheng","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Dentistry School of Dentistry, National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital, College of Medicine, National Taiwan University Taipei Taiwan"},{"name":"Graduate Institute of Oral Biology School of Dentistry, National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi\u2010Feng","family":"Chang","sequence":"additional","affiliation":[{"name":"iStat Biomedical Co, Ltd New Taipei City Taiwan"},{"name":"Academia\u2010Industry Bridging Program (AIBP) National Research Program for Bio\u2010pharmaceuticals Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Hsin","family":"Ko","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Dentistry School of Dentistry, National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital, College of Medicine, National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang\u2010Jaer","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Dentistry School of Dentistry, National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital, College of Medicine, National Taiwan University Taipei Taiwan"},{"name":"Graduate Institute of Oral Biology School of Dentistry, National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin\u2010Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Dentistry School of Dentistry, National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital, College of Medicine, National Taiwan University Taipei Taiwan"},{"name":"Graduate Institute of Oral Biology School of Dentistry, National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huei\u2010Jen","family":"Wang","sequence":"additional","affiliation":[{"name":"iStat Biomedical Co, Ltd New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao\u2010Shan","family":"Lin","sequence":"additional","affiliation":[{"name":"iStat Biomedical Co, Ltd New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6190-7162","authenticated-orcid":false,"given":"Chun\u2010Pin","family":"Chiang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Dentistry School of Dentistry, National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry National Taiwan University Hospital, College of Medicine, National Taiwan University Taipei Taiwan"},{"name":"Graduate Institute of Oral Biology School of Dentistry, National Taiwan University Taipei Taiwan"},{"name":"Department of Dentistry Far Eastern Memorial Hospital New Taipei City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hye138"},{"key":"e_1_2_7_3_1","unstructured":"Health Promotion Administration Ministry of Health and Welfare Taiwan.Cancer Registry Annual Report 2012.http:\/\/www.hpa.gov.tw\/EngPages\/Index.aspx. 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