{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T19:58:51Z","timestamp":1781294331142,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T00:00:00Z","timestamp":1520294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26461043"],"award-info":[{"award-number":["26461043"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A fluorescent probe glutamylprolyl hydroxymethyl rhodamine green (EP\u2010HMRG), which becomes fluorescent after cleavage by dipeptidyl peptidase\u2010IV (DPP\u2010IV), has been reported to be useful for the detection of esophageal cancer. Thus, we investigated whether head and neck squamous cell carcinoma (HNSCC) can be detected by spraying EP\u2010HMRG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fluorescence imaging of 17 cases of HNSCCs resected using endoscopic or surgical resection was performed ex vivo after spraying EP\u2010HMRG, and then the fluorescence intensity of the tumors and normal mucosa were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Iodine\u2010voiding lesions became fluorescent within a few minutes after the application of EP\u2010HMRG in 12 resected tumors without a history of radiotherapy but this was not observed in the normal mucosa. Fluorescence intensity in tumor lesions was significantly higher than normal lesions. However, 5 other tumors that developed after radiotherapy did not have sufficient contrast against normal mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Fluorescence imaging with EP\u2010HMRG would be useful for rapid detection of superficial HNSCC without a history of radiotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.25126","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T14:32:32Z","timestamp":1520346752000},"page":"1466-1475","source":"Crossref","is-referenced-by-count":17,"title":["Rapid detection of superficial head and neck squamous cell carcinoma by topically spraying fluorescent probe targeting dipeptidyl peptidase\u2010IV"],"prefix":"10.1002","volume":"40","author":[{"given":"Takeshi","family":"Mizushima","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Hokkaido University Graduate School of Medicine Sapporo 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