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The CSC markers have been used to isolate these cells and as biomarkers to predict overall survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The CSC markers CD44, NANOG, OCT4, and BMI1 were investigated using reverse transcriptase\u2010quantitative polymerase chain reaction (RT\u2010qPCR) and immunohistochemistry and correlated with clinicopathological parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The CD44 overexpression was associated with disease\u2010related death (<jats:italic>P<\/jats:italic> = 0.02) and worst prognosis. NANOG was upregulated in nontumoral margins and associated with T1\/T2 classification, lymph node metastasis, and worst prognosis. OCT4 was associated with lymph node metastasis and worst overall survival. BMI1 and CD44v3 were overexpressed in tongue SCC. Coexpression of CD44<jats:sup>++<\/jats:sup>\/NANOG<jats:sup>++<\/jats:sup> was associated with worst overall survival when compared with patients with CD44<jats:sup>\u2010\/+<\/jats:sup>\/NANOG<jats:sup>\u2010\/+<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The CSC markers might play an important role not only in CSC trait acquisition but also in tongue SCC development and progression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.25158","type":"journal-article","created":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T06:13:13Z","timestamp":1522649593000},"page":"1759-1773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Prognostic implications of CD44, NANOG, OCT4, and BMI1 expression in tongue squamous cell carcinoma"],"prefix":"10.1002","volume":"40","author":[{"given":"Maria Fernanda Set\u00fabal 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