{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:17:46Z","timestamp":1762431466611,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T00:00:00Z","timestamp":1530144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003500","name":"University of Padova","doi-asserted-by":"publisher","award":["DOR 1658072\/16"],"award-info":[{"award-number":["DOR 1658072\/16"]}],"id":[{"id":"10.13039\/501100003500","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In epithelial\u2010to\u2010mesenchymal transition, epithelial cells lose their features, acquiring a mesenchymal\u2010like phenotype. Nm23\u2010H1 protein relates to tumor cells' metastatic potential, its low expression in carcinomas often meaning a poor prognosis. This study newly investigated the role of nuclear nm23\u2010H1 in laryngeal squamous cell carcinoma epithelial\u2010to\u2010mesenchymal transition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Immunohistochemical analyses of nuclear nm23\u2010H1, E\u2010cadherin, N\u2010cadherin, Snail, Zinc finger E\u2010box binding homeobox (ZEB)1, and ZEB2 were performed in 33 consecutive patients with laryngeal SCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean nuclear nm23\u2010H1 expression was lower in patients whose disease recurred (<jats:italic>P<\/jats:italic> = .0046). Disease\u2010free survival (DFS) was longer for patients whose nuclear nm23\u2010H1 expression was \u226510% (<jats:italic>P<\/jats:italic> = .0083). Nuclear nm23\u2010H1 and E\u2010cadherin expressions correlated directly (<jats:italic>P<\/jats:italic> = .018). Mean E\u2010cadherin expression was lower in patients whose disease recurred (<jats:italic>P<\/jats:italic> = .03). The DFS was shorter in patients with ZEB2 expression \u22655% (<jats:italic>P<\/jats:italic> = .006).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Nuclear nm23\u2010H1 expression warrants further investigation in laryngeal SCC as a prognostic marker identifying patients at higher risk of recurrence. nm23\u2010H1 targeted treatments may be capable of regulating epithelial\u2010to\u2010mesenchymal transition.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.25188","type":"journal-article","created":{"date-parts":[[2018,6,28]],"date-time":"2018-06-28T20:02:01Z","timestamp":1530216121000},"page":"2020-2028","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Nuclear nonmetastatic protein 23\u2010H1 expression and epithelial\u2010mesenchymal transition in laryngeal carcinoma: A pilot 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