{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:57:15Z","timestamp":1792051035634,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T00:00:00Z","timestamp":1544572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30\u2010CA046592R01\u2010CA194536T32 DC535615U01\u2010DE025184"],"award-info":[{"award-number":["P30\u2010CA046592R01\u2010CA194536T32 DC535615U01\u2010DE025184"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01\u2010CA194536"],"award-info":[{"award-number":["R01\u2010CA194536"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30\u2010CA046592"],"award-info":[{"award-number":["P30\u2010CA046592"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01\u2010DE025184"],"award-info":[{"award-number":["U01\u2010DE025184"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32 DC535615"],"award-info":[{"award-number":["T32 DC535615"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010484","name":"American Head and Neck Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We sought to describe targeted DNA sequencing data of persistent\/recurrent laryngeal squamous cell carcinoma (LSCC) and to compare gene\u2010specific alteration frequencies with that of primary, untreated LSCC specimens from The Cancer Genome Atlas (TCGA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The tumors of 21 patients with persistent\/recurrent LSCC were subjected to targeted DNA sequencing using the Ion AmpliSeq Comprehensive Cancer Panel. Gene\u2010specific alteration frequencies were compared (Chi\u2010Square test) to primary, untreated LSCC sequencing data from TCGA using the cBioPortal platform.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Persistent\/recurrent LSCC was characterized by a high rate of inactivating alterations in <jats:italic>TP53<\/jats:italic> (38.1%) and <jats:italic>CDKN2A<\/jats:italic> (33%), amplification events of <jats:italic>CCND1<\/jats:italic> (19.1%), and <jats:italic>ERBB2<\/jats:italic> (14.3%), and <jats:italic>NOTCH1<\/jats:italic> (19.1%) mutations. Comparison of primary vs persistent\/recurrent LSCC revealed significant differences in alteration frequencies of eight critical genes: <jats:italic>BAP1, CDKN2A, DCUN1D1, MSH2, MTOR, PIK3CA, TET2,<\/jats:italic> and <jats:italic>TP53<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results provide preliminary support for a distinct mutational profile of persistent\/recurrent LSCC that requires validation in larger cohorts.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.25444","type":"journal-article","created":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T16:47:08Z","timestamp":1544633228000},"page":"423-428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Mutational profiles of persistent\/recurrent laryngeal squamous cell carcinoma"],"prefix":"10.1002","volume":"41","author":[{"given":"Joshua D.","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew C.","family":"Birkeland","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Rosko","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca C.","family":"Hoesli","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor 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Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven B.","family":"Chinn","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew G.","family":"Shuman","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly M.","family":"Malloy","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith A.","family":"Casper","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"McLean","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory T.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol R.","family":"Bradford","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"},{"name":"Rogel Comprehensive Cancer Center University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Prince","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"},{"name":"Rogel Comprehensive Cancer Center University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3238-1111","authenticated-orcid":false,"given":"John Chad","family":"Brenner","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"},{"name":"Rogel Comprehensive Cancer Center University of Michigan Medical School  Ann Arbor Michigan"},{"name":"Program in Cellular and Molecular Biology University of Michigan Medical School  Ann Arbor Michigan"},{"name":"Department of Pharmacology University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Spector","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of Michigan Medical School  Ann Arbor Michigan"},{"name":"Rogel Comprehensive Cancer Center University of Michigan Medical School  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,12]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Surveillance Epidemiology and End Results (SEER) Program \u2013 National Cancer Institute. 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