{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T14:06:57Z","timestamp":1772806017683,"version":"3.50.1"},"reference-count":67,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A consensus about the most appropriate diagnostic method(s) for head and neck human papillomavirus (HPV)\u2010induced carcinogenesis is still lacking because most of the commercially available assays have been designed for the cervix.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This article summarizes current data and trends concerning HPV diagnostic strategies in oropharyngeal squamous cell carcinoma (OPSCC). Six main approaches are described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The diagnostic gold standard for HPV\u2010related OPSCC, focusing on E6\/E7 mRNA detection, requires fresh samples. Because most frequently available samples are formalin\u2010fixed paraffin\u2010embedded (FFPE), the pros and cons of the different approaches were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In the FFPE samples, the immunohistochemistry of p16, which is considered appropriate to assess HPV\u2010driven carcinogenesis in OPSCC according to the 8th American Joint Committee on Cancer TNM classification, may not be specific enough to become the diagnostic standard in the perspective of treatment deintensification. p16 may play a safer role in combination with another highly sensible assay. Other promising approaches are based on DNA detection through real\u2010time polymerase chain reaction and RNAscope.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.25591","type":"journal-article","created":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T14:36:01Z","timestamp":1549982161000},"page":"1104-1111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["HPV as a marker for molecular characterization in head and neck oncology: Looking for a standardization of clinical use and of detection method(s) in clinical practice"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6261-2772","authenticated-orcid":false,"given":"Francesco","family":"Bussu","sequence":"first","affiliation":[{"name":"Institute of Otolaryngology, Universit\u00e0 Cattolica del Sacro Cuore, Policlinico Agostino Gemelli  Rome Italy"},{"name":"Otolaryngology Division Sassari University Hospital  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille","family":"Ragin","sequence":"additional","affiliation":[{"name":"Cancer Prevention and Control Program, Fox Chase Cancer  Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Boscolo\u2010Rizzo","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, ENT Clinic and Regional Center for Head and Neck Cancer University of Padua, Treviso Regional Hospital  Treviso Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Rizzo","sequence":"additional","affiliation":[{"name":"Otolaryngology Division Sassari University Hospital  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Gallus","sequence":"additional","affiliation":[{"name":"Institute of Otolaryngology, Universit\u00e0 Cattolica del Sacro Cuore, Policlinico Agostino Gemelli  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Delogu","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Universit\u00e0 Cattolica del Sacro Cuore, Policlinico Agostino Gemelli  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Morbini","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Pavia, Policlinico San Matteo  Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Tommasino","sequence":"additional","affiliation":[{"name":"Infections and Cancer Biology Group International Agency for Research on Cancer, World Health Organization  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197605)37:5<2204::AID-CNCR2820370508>3.0.CO;2-E"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2004.10.031"},{"key":"e_1_2_5_4_1","volume-title":"Head and Neck Cancers (Version 2. 2017\u2010May 8, 20117)","author":"National Comprehensive Cancer Network"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10062"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11698"},{"key":"e_1_2_5_7_1","volume-title":"Head and Neck Surgery and Oncology","author":"Shah JP","year":"2003"},{"key":"e_1_2_5_8_1","volume-title":"DeVita, Hellman, and Rosenberg's Cancer: Principles & Practice of Oncology","author":"De Vita V","year":"2008"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.9.709"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(09)70058-9"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0912217"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2014.04.044"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22851"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208130"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-40618-3"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.61.2358"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.05.004"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-43580-0_4"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066693"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-0587"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-013-0509-1"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.63.10.4417-4421.1989"},{"key":"e_1_2_5_23_1","first-page":"101","article-title":"Immortalization and transformation of human cells by human papillomavirus","volume":"186","author":"McDougall JK","year":"1994","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.16.2101"},{"issue":"11","key":"e_1_2_5_25_1","first-page":"2323","article-title":"Inactivation of the cdk inhibitor p27KIP1 by the human papillomavirus type 16 E7 oncoprotein","volume":"13","author":"Zerfass\u2010Thome K","year":"1996","journal-title":"Oncogene"},{"issue":"6","key":"e_1_2_5_26_1","first-page":"2356","article-title":"Human papillomavirus type 16 E7 oncoprotein\u2010induced abnormal centrosome synthesis is an early event in the evolving malignant phenotype","volume":"61","author":"Duensing S","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.1.463"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.1.473-478.1991"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.55"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318253a2d1"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-0388"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22980"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2009.08.018"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.00.3335"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016480701558880"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20175"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.23.1670"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63871-0"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21974"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semradonc.2012.03.003"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.09.015"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt053"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-14-1217"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.47.2738"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-3277"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3812"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01944.x"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/314111a0"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2013.11.002"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208619"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310432110"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03817-13"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28900"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2015.146"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.36.4596"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23866"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2012.07.002"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.55.1341"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2015.2524"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2013.54.5228"},{"issue":"5","key":"e_1_2_5_61_1","first-page":"1203","article-title":"Real\u2010time quantitative PCR demonstrates low prevalence of human papillomavirus type 16 in premalignant and malignant lesions of the oral cavity","volume":"8","author":"Ha PK","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv403"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.296"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.63"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.113"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2014.11.011"},{"key":"e_1_2_5_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27821"},{"key":"e_1_2_5_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2016.10.009"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhed.25591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.25591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hed.25591","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.25591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T20:39:43Z","timestamp":1694119183000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.25591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,12]]},"references-count":67,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/hed.25591"],"URL":"https:\/\/doi.org\/10.1002\/hed.25591","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,12]]},"assertion":[{"value":"2018-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}