{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:49:00Z","timestamp":1780505340753,"version":"3.54.1"},"reference-count":12,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:00:00Z","timestamp":1562716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The purpose of this study was to determine the expression profile of several biomarkers in sestamibi\u2010positive (<jats:italic>n<\/jats:italic> =\u200923) and sestamibi\u2010negative (<jats:italic>n<\/jats:italic> = 6) parathyroid adenomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A tissue microarray of parathyroid adenomas from 29 patients was constructed and slides were stained for several proteins involved in angiogenesis, inflammation, cell adhesion, cell cycle, apoptosis, and with markers of the sonic hedgehog, mTOR, Forkhead box O and WNT signal transduction pathways. Protein expression was determined using an image\u2010analysis software (Spectrum Plus\u00a9, 38 Aperio).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Protein expression analysis revealed that the vascular endothelial growth factor receptor 2 (VEGFR2) score was significantly higher in the sestamibi\u2010positive cohort compared to sestamibi\u2010negative adenomas (<jats:italic>P<\/jats:italic> =\u2009.038). Other proteins were not differentially expressed between sestamibi\u2010positive and sestamibi\u2010negative adenomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is hypothesized that VEGFR\u20102 overexpression in parathyroid adenomas increases vascular permeability resulting in a higher uptake of sestamibi.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.25871","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T14:13:53Z","timestamp":1562768033000},"page":"3564-3569","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["VEGFR\u20102 is downregulated in sestamibi\u2010negative parathyroid adenomas"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5706-1056","authenticated-orcid":false,"given":"Boban M.","family":"Erovic","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"},{"name":"Department of Surgical Oncology Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"},{"name":"Institute of Head and Neck Diseases Evangelical Hospital Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P.","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"},{"name":"Department of Surgical Oncology Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia L.","family":"Asa","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1161-4871","authenticated-orcid":false,"given":"Stefan","family":"Janik","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozgur","family":"Mete","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan C.","family":"Irish","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"},{"name":"Department of Surgical Oncology Princess Margaret Hospital\/University Health Network, University of Toronto  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1475-097X.2011.01048.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07435800.2018.1437747"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006544"},{"key":"e_1_2_5_5_1","first-page":"1","article-title":"Combined ultrasound and sestamibi scintigraphy provides accurate preoperative localisation for patients with primary hyperparathyroidism","volume":"5","author":"Scattergood S","year":"2018","journal-title":"Ann R Coll Surg Engl"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24027"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-012-9222-y"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2008.03.019"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12149-018-1239-y"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006231-200302000-00003"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.13.3898"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.151019"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11017"}],"container-title":["Head &amp; 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