{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:11:29Z","timestamp":1790997089460,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005440","name":"Center for Scientific Review","doi-asserted-by":"publisher","award":["P30 CA069533"],"award-info":[{"award-number":["P30 CA069533"]}],"id":[{"id":"10.13039\/100005440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005440","name":"Center for Scientific Review","doi-asserted-by":"publisher","award":["U54CA112970"],"award-info":[{"award-number":["U54CA112970"]}],"id":[{"id":"10.13039\/100005440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["S10\u2010RR023432"],"award-info":[{"award-number":["S10\u2010RR023432"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Levels of circulating hybrid cells (CHCs), a newly identified circulating tumor cell (CTC), correlate with disease stage and progression in cancer. We investigated their utility to risk\u2010stratify patients with clinically N0 (cN0) oral cavity squamous cell carcinoma (OCSCC), and to identify patients with occult cervical lymph node metastases (pN+).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed peripheral blood samples for CHCs with co\u2010expression of cytokeratin (tumor) and CD45 (leukocyte) from 22 patients with cN0 OCSCC using immunofluorescence microscopy, then correlated levels with pathologic lymph node status.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CHC levels exceeded CTCs and correlated with the presence of both clinically overt (<jats:italic>p<\/jats:italic>\u00a0=\u20090.002) and occult nodal metastases (<jats:italic>p<\/jats:italic>\u00a0=\u20090.006).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For evaluated cN0 OCSCC patients, those with cN0\u2009\u2192\u2009pN+ status harbored elevated CHC levels compared to patients without occult disease. Our findings highlight a promising blood\u2010based biologic assay with potential utility to determine the necessity of surgical neck dissection for staging and treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.26692","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T13:04:01Z","timestamp":1617973441000},"page":"2193-2201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Circulating hybrid cells predict presence of occult nodal metastases in oral cavity carcinoma"],"prefix":"10.1002","volume":"43","author":[{"given":"Tara E.","family":"Henn","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley N.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Cell, Developmental and Cancer Biology Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvette R.","family":"Hollett","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Sutton","sequence":"additional","affiliation":[{"name":"Department of Surgery Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett S.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Surgery Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Swain","sequence":"additional","affiliation":[{"name":"Department of Cell, Developmental and Cancer Biology Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Sauer","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Oregon Health &amp; Science University  Portland Oregon USA"},{"name":"Department of Pathology Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3296-4926","authenticated-orcid":false,"given":"Daniel R.","family":"Clayburgh","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Oregon Health &amp; Science University  Portland Oregon USA"},{"name":"Operative Care Division Veterans Affairs Medical Center  Portland Oregon USA"},{"name":"Knight Cancer Institute Oregon Health &amp; Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5127-279X","authenticated-orcid":false,"given":"Melissa H.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Cell, Developmental and Cancer Biology Oregon Health &amp; Science University  Portland Oregon USA"},{"name":"Knight Cancer Institute Oregon Health &amp; 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