{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:34:08Z","timestamp":1787168048386,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T00:00:00Z","timestamp":1627862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007137","name":"Helse Nord RHF","doi-asserted-by":"publisher","award":["SFP1276\u201016"],"award-info":[{"award-number":["SFP1276\u201016"]}],"id":[{"id":"10.13039\/501100007137","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005366","name":"Universitetet i Oslo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to evaluate the prognostic significance of expression levels of involucrin (IVL), cytokeratin (CK)\u201010 and \u201013 at different intratumor sites (tumor center and invading area) of oral tongue squamous cell carcinoma (OTSCC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>IVL, CK13 and CK10 expression levels were examined in a multicenter cohort of 146 OTSCCs using immunohistochemistry. External mRNA datasets were used for expression analysis and\/or to validate survival associations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      External transcriptomic datasets showed downregulation of\n                      <jats:italic>IVL<\/jats:italic>\n                      and\n                      <jats:italic>KRT13<\/jats:italic>\n                      in oral malignancies including OTSCC as compared to normal controls. The combined loss of IVL and CK13 expression at the invading core but not at the center core was significantly associated with poor differentiation and reduced 5\u2010year overall survival. Multivariate Cox analysis confirmed the loss of CK13 and IVL expression to be an independent prognostic factor. Transcriptomic dataset corroborated immunohistochemistry results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Combined expression levlels of IVL and CK13 might be useful as prognostic biomarkers in OTSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hed.26826","type":"journal-article","created":{"date-parts":[[2021,8,2]],"date-time":"2021-08-02T08:29:10Z","timestamp":1627892950000},"page":"3374-3385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Combined loss of expression of involucrin and cytokeratin 13 is associated with poor prognosis in squamous cell carcinoma of mobile tongue"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5483-1577","authenticated-orcid":false,"given":"Sushma","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1687-3247","authenticated-orcid":false,"given":"Tine Merete","family":"S\u00f8land","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"},{"name":"Department of Pathology Oslo University Hospital, Rikshospitalet  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inger Heidi","family":"Bjerkli","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology University Hospital of North Norway  Troms\u00f8 Norway"},{"name":"Department of Troms\u00f8 Medical Biology, Faculty of Health Sciences UiT \u2013 The Arctic University of Norway  Troms\u00f8 Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars Peter","family":"Sand","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Cristina","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7673-0358","authenticated-orcid":false,"given":"Daniela Elena","family":"Costea","sequence":"additional","affiliation":[{"name":"The Gade Laboratory for Pathology, Department of Clinical Medicine Haukeland University Hospital, University of Bergen  Bergen Norway"},{"name":"Department of Pathology Haukeland University Hospital  Bergen Norway"},{"name":"Centre for Cancer Biomarkers (CCBIO), Faculty of Medicine and Dentistry University of Bergen  Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcu","family":"Senguven","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"},{"name":"Department of Oral Pathology, Faculty of Dentistry Gazi University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0061-825X","authenticated-orcid":false,"given":"Dipak","family":"Sapkota","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0227738"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6831-14-103"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coms.2014.01.001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2017.09.021"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.20462"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2010.06.006"},{"key":"e_1_2_9_8_1","volume-title":"WHO Classification of Head and Neck Tumours","author":"El\u2010Naggar AC J","year":"2017"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.22015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn011"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14129"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.04.004"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-015-0219-2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03759.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2011.03.015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1986.tb00608.x"},{"issue":"2","key":"e_1_2_9_17_1","first-page":"215","article-title":"Loss of cytokeratin 13 expression in squamous cell carcinoma of the tongue is a possible sign for local recurrence","volume":"26","author":"Yanagawa T","year":"2007","journal-title":"J Exp Clin Cancer Res"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/coa.13092"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-07-2893"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1262110"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx247"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0095"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2004088"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.342"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/b137945"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001216"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90400-7"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118702840"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.2209\/tdcpublication.56.223"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1984.00800370015003"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(87)90011-9"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.218"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1991.tb00430.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2018.12.007"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-988"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1993.tb01182.x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(00)00094-4"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp-journals-10024-1502"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110040101"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-020-02875-9"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238103"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.2880120604"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-1-113"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.26826","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hed.26826","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.26826","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T16:57:53Z","timestamp":1692982673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.26826"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,2]]},"references-count":43,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1002\/hed.26826"],"URL":"https:\/\/doi.org\/10.1002\/hed.26826","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,2]]},"assertion":[{"value":"2021-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}