{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:54:17Z","timestamp":1791986057982,"version":"4.4.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,24]],"date-time":"2021-10-24T00:00:00Z","timestamp":1635033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","award":["2017R1D1A1B03029860"],"award-info":[{"award-number":["2017R1D1A1B03029860"]}],"id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cutaneous angiosarcoma (CAS) is a rare but fatal cancer. Established CAS cell lines are necessary for the investigation of their properties and treatment options.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two cell lines, KU\u2010CAS3 and KU\u2010CAS5, were established from human angiosarcoma specimens obtained from the scalp. Flow cytometric assay, tube formation assay, low\u2010density lipoprotein (LDL) uptake assay, immunofluorescence analysis, real\u2010time PCR, tumorigenesis assay, and STR analysis were conducted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The cells showed endothelial cell properties, based on the cobblestone appearance upon reaching confluence, CD31 positivity, tube\u2010formation activity, active uptake of acetylated LDL, and vWF expression. The two cell lines expressed relatively high levels of adrenergic \u03b22 receptor, and the VEGF1 and VEGF2 receptors. In the in vivo study, the growing neoplasms, confirmed as CAS, were identified as subcutaneous dark papules. KU\u2010CAS cell lines were considered authentic based on STR profiling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>KU\u2010CAS3 and KU\u2010CAS5 are the first human CAS cell lines having tumorigenic potential in vivo.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.26886","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T06:18:07Z","timestamp":1635142687000},"page":"7-17","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Establishment and characterization of two human cutaneous angiosarcoma cell lines, <scp>KU\u2010CAS3<\/scp> and <scp>KU\u2010CAS5<\/scp>"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9997-2736","authenticated-orcid":false,"given":"Hi\u2010Jin","family":"You","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung\u2010Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Sook","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Hwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Ho","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na\u2010Hyun","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deok\u2010Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung\u2010Woo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju\u2010Han","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology Korea University Medicine  Ansan South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCO.0000000000000427"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990412)81:2<305::AID-IJC22>3.0.CO;2-Z"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10623320390272316"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2013.02.008"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11252"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.m600292-jlr200"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00046"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00130404-200505000-00011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-013-0328-2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.141"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010515)91:10<1914::AID-CNCR1214>3.0.CO;2-3"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021573013503"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci107470"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.ccr-17-1778"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0923-1811(97)00032-7"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.6.2034"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0530291100"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.26886","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hed.26886","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.26886","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T05:05:04Z","timestamp":1692507904000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.26886"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,24]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/hed.26886"],"URL":"https:\/\/doi.org\/10.1002\/hed.26886","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,24]]},"assertion":[{"value":"2021-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}