{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T12:15:47Z","timestamp":1776082547991,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T00:00:00Z","timestamp":1666656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["02NUK032"],"award-info":[{"award-number":["02NUK032"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018515","name":"Hamburger Krebsgesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We have recently shown a frequent upregulation of Src\u2010family kinases (SFK) in head and neck squamous cell carcinoma (HNSCC). Here we tested, if SFK targeting is effective especially in HNSCC cells with upregulated SFK signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The impact of SFK inhibitors SU6656, PP2 and dasatinib on three HNSCC cell lines with different SFK activity levels was analyzed using proliferation and colony formation assays, Western blot and functional kinomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Proliferation was blocked by all inhibitors in a micro\u2010molar range. With respect to cell kill, dasatinib was most effective, while SU6656 showed moderate and PP2 minor effects. Cellular signaling was affected differently, with PP2 having no effect on SFK signaling while dasatinib probably has non\u2010SFK specific effects. Only SU6656 showed clear SFK specific effects on signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results demonstrate potential benefit of SFK inhibition in HNSCC but they also highlight challenges due to non\u2010specificities of the different drugs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.27216","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T06:18:02Z","timestamp":1666765082000},"page":"147-155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Src family kinase targeting in head and neck tumor cells using <scp>SU6656<\/scp>, <scp>PP2<\/scp> and dasatinib"],"prefix":"10.1002","volume":"45","author":[{"given":"Anh Thu","family":"Vu","sequence":"first","affiliation":[{"name":"Department of Radiobiology &amp; Radiation Oncology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Akingunsade","sequence":"additional","affiliation":[{"name":"Department of Radiobiology &amp; Radiation Oncology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin","family":"Hoffer","sequence":"additional","affiliation":[{"name":"Department of Radiobiology &amp; Radiation Oncology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"UCCH Kinomics Core Facility, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cordula","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Radiobiology &amp; Radiation Oncology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian Stephan","family":"Betz","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Rothkamm","sequence":"additional","affiliation":[{"name":"Department of Radiobiology &amp; Radiation Oncology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8426-107X","authenticated-orcid":false,"given":"Thorsten","family":"Rieckmann","sequence":"additional","affiliation":[{"name":"Department of Radiobiology &amp; Radiation Oncology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Department of Otorhinolaryngology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Bussmann","sequence":"additional","affiliation":[{"name":"UCCH Kinomics Core Facility, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Department of Otorhinolaryngology, Hubertus Wald Tumorzentrum \u2013 University Cancer Center Hamburg (UCCH) University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Mildred Scheel Cancer Career Center HaTriCS4 University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6195-8920","authenticated-orcid":false,"given":"Malte","family":"Kriegs","sequence":"additional","affiliation":[{"name":"Department of Radiobiology &amp; 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