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Using these models, this study examined the therapeutic effect of human epidermal growth factor receptor 2 (HER2) blockade on HER2\u2010positive SDC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The therapeutic effect of lapatinib was assessed in SDC PDXOs with regards to cell growth, receptor\/downstream signaling molecule expression, phosphorylation levels, and apoptosis. Effect of lapatinib treatment was evaluated in vivo in SDC PDX mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The siRNA knockdown of HER2 and lapatinib suppressed cell proliferation in SDC PDXOs. Lapatinib inhibited the phosphorylation of HER2 and its downstream targets, and induced apoptosis in SDC PDXOs. Lapatinib also significantly reduced tumor volumes compared with that of the control in SDC PDX mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>For the first time, we demonstrated the efficacy of anti\u2010HER2 therapy in HER2\u2010positive SDC using preclinical models of SDC PDX and PDXO.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.27395","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T14:29:16Z","timestamp":1684160956000},"page":"1801-1811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of <scp>HER2<\/scp>\u2010targeted therapy on <scp>PDX<\/scp> and <scp>PDX<\/scp>\u2010derived organoids generated from <scp>HER2<\/scp>\u2010positive salivary duct carcinoma"],"prefix":"10.1002","volume":"45","author":[{"given":"Jun","family":"Aoyama","sequence":"first","affiliation":[{"name":"Department of Biology and Function in Head and Neck Yokohama City University Graduate School of Medicine  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Nojima","sequence":"additional","affiliation":[{"name":"Department of Biology and Function in Head and Neck Yokohama City University Graduate School of Medicine  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7686-4724","authenticated-orcid":false,"given":"Daisuke","family":"Sano","sequence":"additional","affiliation":[{"name":"Department of Biology and Function in Head and Neck Yokohama City University Graduate School of Medicine  Yokohama Japan"},{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Yokohama City University, School of Medicine  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Hirai","sequence":"additional","affiliation":[{"name":"Department of Biology and Function in Head and Neck Yokohama City 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