{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:39:34Z","timestamp":1789205974414,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Treatment of salivary gland tumors (SGTs) remains challenging. Little is known about the immune landscape of SGTs. We aimed to characterize the tumor microenvironment in benign and malignant SGTs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eleven benign and nine malignant tumors were collected from patients undergoing curative intent surgery. Specimens were analyzed using mass cytometry by time\u2010of\u2010flight. Immune cell populations were manually gated, and T cells were clustered using the <jats:italic>FlowSOM<\/jats:italic> algorithm. Population frequencies were compared between high\u2010grade and low\u2010grade malignancies, corrected for multiple hypothesis testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were trends towards increased CD4+ and CD8+ T cells among malignant tumors. High\u2010grade malignancies exhibited trends towards higher frequencies of CD8+ PD\u20101+ CD39+ CD103+ exhausted T cells, CD4+ FoxP3+ TCF\u20101+ CD127\u2212 Tregs, and CD69+ CD25\u2212 CD4+ T cells compared to low\u2010grade malignancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SGTs exhibit significant immunologic diversity. High\u2010grade malignancies tended to have greater infiltration of exhausted CD8+ T cells and Tregs, which may guide future studies for immunotherapy strategies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.27716","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T06:04:17Z","timestamp":1709877857000},"page":"1625-1636","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The tumor microenvironment of benign and malignant salivary gland tumors"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8180-4542","authenticated-orcid":false,"given":"Katherine C.","family":"Wai","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Department of Microbiology and Immunology University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine Line H.","family":"Okholm","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Department of Microbiology and Immunology University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick K.","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana M.","family":"Marquez","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Department of Microbiology and Immunology University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iliana","family":"Tenvooren","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Department of Microbiology and Immunology University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle B.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Department of Microbiology and Immunology University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"},{"name":"Department of Orofacial Sciences University of California San Francisco  San Francisco California USA"},{"name":"Pharma Technical Cell and Gene Therapy, Genentech, Inc.  South San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew H.","family":"Spitzer","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery University of California San Francisco  San Francisco California USA"},{"name":"Department of Microbiology and Immunology University of California San Francisco  San Francisco California USA"},{"name":"Helen Diller Family Comprehensive Cancer Center University of California San Francisco  San Francisco California USA"},{"name":"Parker Institute for Cancer Immunotherapy  San Francisco California USA"},{"name":"Chan Zuckerberg Biohub  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-3284-4-19"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rpor.2018.05.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1602252"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/COC.0000000000000429"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-022-01486-x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.701181"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-3758"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S134589"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.04.019"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0892-6"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.12.022"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.02.018"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.02.021"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1259425"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.020"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23904"},{"key":"e_1_2_6_18_1","unstructured":"EllisBHP HahneN Le MeurN et al.flowCore: basic structures for flow cytometry data; 2022."},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22625"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.11622.1"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2018.02.010"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq170"},{"key":"e_1_2_6_23_1","unstructured":"McInnesL HealyJ.UMAP: uniform manifold approximation and projection for dimension reduction. 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