{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:46:46Z","timestamp":1788576406738,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T00:00:00Z","timestamp":1711324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008363","name":"Kr\u00e6ftens Bek\u00e6mpelse","doi-asserted-by":"publisher","award":["R231\u2010A14155"],"award-info":[{"award-number":["R231\u2010A14155"]}],"id":[{"id":"10.13039\/100008363","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008363","name":"Kr\u00e6ftens Bek\u00e6mpelse","doi-asserted-by":"publisher","award":["R146\u2010A9519"],"award-info":[{"award-number":["R146\u2010A9519"]}],"id":[{"id":"10.13039\/100008363","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In head and neck squamous cell carcinomas (HNSCC), there is no clinically available method to separate distant metastases (DMs) from SCC secondary primary tumors. The study aimed to assess the genetic relationship in paired tumor samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with pairs of solid biopsies from the primary HNSCC and suspected DMs were identified (2007\u20132017). Targeted next\u2010generation sequencing of 22 genes was applied, including <jats:italic>TP53<\/jats:italic>, supplemented with human papillomavirus (HPV) genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 55 pairs obtained, 33 were successfully analyzed. Distant biopsies included lung, liver, and bone. A genetic match was found in 23\/33 (70%) patients, primarily with identical <jats:italic>TP53<\/jats:italic> mutations or HPV genotypes. In 10\/33 patients (30%), the genetic relationship was absent, all with lung involvement. In patients with no lung involvement, 8\/8 had a match.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>One\u2010third of patients with DMs in HNSCC lack a genetic relationship with the primary tumors. The risk of misclassification is most prominent for patients with lung involvement.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.27750","type":"journal-article","created":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T06:55:28Z","timestamp":1711436128000},"page":"2532-2539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Separating distant recurrences from second primaries in head and neck squamous cell carcinomas \u2013 A <scp>DAHANCA<\/scp> group analysis on paired tumor samples"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0274-6960","authenticated-orcid":false,"given":"Julie","family":"Kjems","sequence":"first","affiliation":[{"name":"Department of Experimental Clinical Oncology Aarhus University Hospital  Aarhus Denmark"},{"name":"Department of Oncology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6400-6650","authenticated-orcid":false,"given":"Jacob Kinggaard","family":"Lilja\u2010Fischer","sequence":"additional","affiliation":[{"name":"Department of Experimental Clinical Oncology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5423-0627","authenticated-orcid":false,"given":"Jeppe","family":"Friborg","sequence":"additional","affiliation":[{"name":"Department of Oncology Copenhagen University Hospital  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3894-4552","authenticated-orcid":false,"given":"Trine","family":"Tramm","sequence":"additional","affiliation":[{"name":"Department of Pathology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-8179","authenticated-orcid":false,"given":"Jens","family":"Overgaard","sequence":"additional","affiliation":[{"name":"Department of Experimental Clinical Oncology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2021.1959056"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1503\/CMAJ.110167"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201005\u20100257"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/IJC.23798"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/LARY.27422"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.25041"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2023.2260944"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20100142(20000215)88:4<884::AID\u2010CNCR20>3.0.CO;2\u2010S"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2019.1629015"},{"key":"e_1_2_10_11_1","unstructured":"The Danish Pathology Register. Accessed November 14 2023.https:\/\/ncrr.au.dk\/danish-registers\/the-danish-pathology-register"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2023.2266127"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201008\u20101968"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2018.03.063"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/NATURE14129"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/ONCOTARGET.11196"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/MC.23385"},{"key":"e_1_2_10_18_1","unstructured":"COSMIC \u2013 Catalogue of Somatic Mutations in Cancer. 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