{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:39:25Z","timestamp":1792514365304,"version":"4.5.0"},"reference-count":19,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp;amp; Neck"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The purpose of this multicenter study was to retrospectively investigate the prognostic significance of the tumor microenvironment, in relation to survival in a large cohort of patients with laryngeal squamous cell carcinoma (LSCC), using the method proposed by the International TILs Working Group in breast cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>All consecutive patients with biopsy\u2010proven LSCC who underwent total laryngectomy (TL) between January 2014 and January 2023 were retrospectively included in the study. A retrospective review of medical records including surgical, pathological and follow\u2010up reports was performed. The density of TILs was determined according to the recommendations of the International TILs Working Group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The study group included 186 patients with LSCC. High TILs were statistically correlated with reduced size and extension of primary tumor (pT stage) with a statistically significant value (S:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01; P:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0003) and without needs of salvage therapy (S:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.03; P:\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.004). Low TILs were indicative of worse prognosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study confirmed the protective value of TILs and the prognostic role of the tumor microenvironment in LSCC; furthermore, our results showed that the score proposed by the International TILs Working Group for breast cancer can be applied to LSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hed.27860","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T07:16:01Z","timestamp":1719990961000},"page":"2123-2131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A multicenter protocol to assess the prognostic significance of the tumor microenvironment in patients with squamous cell carcinoma of the larynx"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6505-1680","authenticated-orcid":false,"given":"Pietro","family":"De Luca","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Isola Tiberina \u2013 Gemelli Isola Hospital  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Di Stadio","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology University of Catania  Catania Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0371-7667","authenticated-orcid":false,"given":"Gerardo","family":"Petruzzi","sequence":"additional","affiliation":[{"name":"Department Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"de Campora","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology San Giovanni\u2010Addolorata Hospital  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Fior","sequence":"additional","affiliation":[{"name":"Department Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Moretti","sequence":"additional","affiliation":[{"name":"Department Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Della Peruta","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery AORN Ospedali dei Colli Monaldi \u2013 Cotugno \u2013 C.T.O. Hospital  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Mazzola","sequence":"additional","affiliation":[{"name":"Department Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopoldo","family":"Costarelli","sequence":"additional","affiliation":[{"name":"Division of Pathology Azienda Ospedaliera San Giovanni\u2010Addolorata  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Covello","sequence":"additional","affiliation":[{"name":"Department of Pathology IRCCS Regina Elena National Cancer Institute  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Ricciardiello","sequence":"additional","affiliation":[{"name":"Otolaryngology Unit AORN Cardarelli  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Tortoriello","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery AORN Ospedali dei Colli Monaldi \u2013 Cotugno \u2013 C.T.O. Hospital  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Pellini","sequence":"additional","affiliation":[{"name":"Department Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Radici","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Isola Tiberina \u2013 Gemelli Isola Hospital  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Camaioni","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology San Giovanni\u2010Addolorata Hospital  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2021.151787"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432\u2010019\u201002936\u2010w"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010019\u201001101\u20106"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04169.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.29196"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9050486"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262\u2010018\u20102256\u20103"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu450"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912\u2010019\u20100799\u2010x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010017\u20100788\u2010z"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0344\u20100338(11)80700\u20104"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192744"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal5081"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00199380"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199707)182:3<318::AID-PATH862>3.0.CO;2-6"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13040781"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/01945998211013765"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2017.12.003"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.682435"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.27860","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hed.27860","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.27860","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:28:40Z","timestamp":1772983720000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.27860"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,3]]},"references-count":19,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/hed.27860"],"URL":"https:\/\/doi.org\/10.1002\/hed.27860","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,3]]},"assertion":[{"value":"2024-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}