{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:28:40Z","timestamp":1789194520506,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp;amp; Neck"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>First aim was to compare ddPCR assays of ctHPVDNA with p16 IHC and qualitative HPV PCR. Second aim was to carry out longitudinal blood sampling to test for association of ctHPVDNA with histological confirmed recurrence. Third aim was to perform a multidimensional assessment which included: (1) clinical features; (2) ctHPVDNA; (3) MRI\u2010based tumor size measurements of primary tumor (PT) and cervical lymph node metastases (CLNM).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Plasma samples were collected before treatment and during follow\u2010up, and ddPCR assay comprising E6 of HPV16 and HPV 33 and HPV 35 was used.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Present study was conducted at diagnosis in 117 patients and revealed a ctHPVDNA sensitivity of 100% (95% CI 95.5\u2013100) and a specificity of 94.4 (95% CI 81.3\u201399.3), positive predictive value (PPV) of 94.4 (95% CI 81.3\u201399.3), and negative predictive value (NPP) of 100% (95% CI 89.7\u2013100). During follow\u2010up ctHPVDNA had a sensitivity of 100% (95% CI 72.1\u2013100)% and specificity of 98.4% (95% CI 91.7\u2013100)%, PPV% of 90.9% (95% CI 62.3\u201398.4) and NPV% of 100% (95% CI 94.3\u2013100) for ability to detect recurrence. Correlation between both the CLNM volume and the sum of PT and CLNM volume was observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ctHPVDNA was superior to p16 in identification of HPV\u2010OPSCC at diagnosis. Introduction of ctHPVDNA, beyond diagnostic setting, represents a great opportunity to improve follow\u2010up protocol of OPSCC patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hed.27866","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T07:19:16Z","timestamp":1720509556000},"page":"2206-2213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Circulating tumor\n                    <scp>HPV DNA<\/scp>\n                    in the management of\n                    <scp>HPV<\/scp>\n                    + oropharyngeal cancer and its correlation with\n                    <scp>MRI<\/scp>"],"prefix":"10.1002","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9249-7573","authenticated-orcid":false,"given":"Flaminia","family":"Campo","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Paolini","sequence":"additional","affiliation":[{"name":"HPV\u2010Unit, UOSD Tumor Immunology and Immunotherapy, IRCCS Regina Elena National Cancer Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"},{"name":"Department of Biochemical Sciences A. Rossi Fanelli Sapienza University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Manciocco","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Moretto","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Pichi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Moretti","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery IRCCS Regina Elena National Cancer Institute, Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Blandino","sequence":"additional","affiliation":[{"name":"Oncogenomic and Epigenetic Unit, IRCCS Regina Elena National Cancer Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"De Pascale","sequence":"additional","affiliation":[{"name":"Translational Oncologic Research, IRCCS Regina Elena National Cancer Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Benevolo","sequence":"additional","affiliation":[{"name":"Department of Pathology IRCCS Regina Elena National Cancer Institute  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulvia","family":"Pimpinelli","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, IRCCS San Gallicano Dermatological Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonello","family":"Vidiri","sequence":"additional","affiliation":[{"name":"Department of Radiology and Diagnostic Imaging, IRCCS Regina Elena National Cancer Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Marzi","sequence":"additional","affiliation":[{"name":"Department of Radiology and Diagnostic Imaging, IRCCS Regina Elena National Cancer Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ruggiero","sequence":"additional","affiliation":[{"name":"Department of Radiology and Diagnostic Imaging, IRCCS Regina Elena National Cancer Institute Istituti Fisioterapici Ospitalieri (IFO)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Terrenato","sequence":"additional","affiliation":[{"name":"Clinical Trial Center \u2013 Biostatistics &amp; 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