{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T19:58:04Z","timestamp":1780343884263,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009812","name":"Princess Margaret Cancer Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009812","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Radiotherapy (RT) plays a crucial role in head and neck (HN) cancer treatment. Nevertheless, it can lead to serious and challenging adverse events such as osteoradionecrosis (ORN). A preclinical rabbit model of irradiated bone and ORN is herein proposed, with the aim to develop a viable model to be exploited for investigating new therapeutic approaches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nine New Zealand white rabbits were irradiated using a single beam positioned to the left of the mandible and directed perpendicular to the left mandible. A 10\u2009\u00d7\u200910\u2009mm<jats:sup>2<\/jats:sup> region of interest (ROI) located below the first molar tooth on the left side was identified and irradiated with 7\u2009Gy each fraction, once every 2\u2009days, for five fractions. Dose distributions demonstrated that the corresponding ROI on the contralateral (right) mandibular side received approximately 5\u2009Gy each fraction, thus bilateral irradiation of the mandible was achieved. ROIs were categorized as ROI<jats:sub>H<\/jats:sub> on the left side receiving the high dose and ROI<jats:sub>L<\/jats:sub> on the right side receiving the low dose. Rabbits were followed up clinically and imaged monthly. After 4\u2009months, the irradiated bone was excised, and histological examination of ROIs was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Radiological signs suggestive for ORN were detected in the entire population (100%) 16\u2009weeks after irradiation on ROI<jats:sub>H<\/jats:sub>, which consisted of cortical erosion and loss of trabeculae. ROI<jats:sub>L<\/jats:sub> did not show any radiological evidence of bone damage. Histologically, both sides showed comparable signs of injury, with marked reduction in osteocyte count and increase in empty lacunae count.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A preclinical double model was successfully developed. The side receiving the higher dose showed radiological and histological signs of bone damage, resulting in an ORN model. Whereas the contralateral side, receiving the lower dose, presented with histological damage only and a normal radiological appearance. This work describes the creation of a double model, an ORN and irradiated bone model, for further study using this animal species.<\/jats:p><\/jats:sec>","DOI":"10.1002\/hed.27955","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T04:13:51Z","timestamp":1728015231000},"page":"625-634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a Preclinical Double Model of Mandibular\u00a0Irradiated Bone and Osteoradionecrosis in New\u00a0Zealand Rabbits"],"prefix":"10.1002","volume":"47","author":[{"given":"Alessandra","family":"Ruaro","sequence":"first","affiliation":[{"name":"Guided Therapeutics (GTx) Program, International Scholarship Program, Techna Institute University Health Network  Toronto Ontario Canada"},{"name":"Section of Otorhinolaryngology\u2014Head and Neck Surgery, Department of Neurosciences University of Padova  Padova Italy"},{"name":"Unit of Otorhinolaryngology\u2014Head and Neck Surgery Azienda Ospedale Universit\u00e0 di Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2407-4031","authenticated-orcid":false,"given":"Stefano","family":"Taboni","sequence":"additional","affiliation":[{"name":"Guided Therapeutics (GTx) Program, International Scholarship Program, Techna Institute University Health Network  Toronto Ontario Canada"},{"name":"Section of Otorhinolaryngology\u2014Head and Neck Surgery, Department of Neurosciences University of Padova  Padova Italy"},{"name":"Unit of Otorhinolaryngology\u2014Head and Neck Surgery Azienda Ospedale Universit\u00e0 di Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harley H. 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