{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:57:03Z","timestamp":1791118623290,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":6164,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We studied epidermal growth factor receptor (EGFR) gene am plification and expression in 11 early passage human head and neck carcinoma cell lines. Three cell lines demonstrated EGFR gene amplification and 10 lines showed an increase in EGFR mRNA when compared with normal keratinocytes, placenta, and a human skin carcinoma cell line. The effects of EGF on growth in 6 head and neck carcinoma cell lines was also studied. Growth inhibition at a concentration of 20 ng\/mL was observed in one cell line but had no effect on growth in 5 cell lines. An increase in EGFR may be important in the etiology of, or progression of, head and neck carcinoma although the mecha nisms need to be elucidated by further study.<\/jats:p>","DOI":"10.1002\/hed.2880110510","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T16:08:12Z","timestamp":1172074092000},"page":"437-442","source":"Crossref","is-referenced-by-count":62,"title":["Epidermal growth factor receptor gene amplification and expression in head and neck cancer cell lines"],"prefix":"10.1002","volume":"11","author":[{"given":"Ralph R.","family":"Weichselbaum","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J.","family":"Dunphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Beckett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen G.","family":"Tybor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Everett E.","family":"Vokes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Panje","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/311477a0"},{"key":"e_1_2_1_3_2","first-page":"729","article-title":"Modulation of the epidermal growth factor receptor by mitogenic ligands: Effects of bombesin and the role of protein kinase C. 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Growth factors and malignancy","volume":"4","author":"Zachary I","year":"1985","journal-title":"Cancer Surv"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/307521a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/309418a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/313144a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6200934"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/293305a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01534689"},{"key":"e_1_2_1_10_2","first-page":"41","volume-title":"Cancer cells growth factors and transformation","author":"Ozanne B","year":"1985"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00591a005"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/309806a0"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"e_1_2_1_14_2","volume-title":"Molecular cloning: A laboratory manual","author":"Manaiatis T","year":"1982"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711490104"},{"key":"e_1_2_1_16_2","first-page":"414","article-title":"High inci dence of amplification of the epidermal growth factor re ceptor gene in human squamous cell carcinoma cell lines","volume":"46","author":"Yamamoto T","year":"1986","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711520408"},{"key":"e_1_2_1_18_2","first-page":"3603","article-title":"Analysis of the epi dermal growth factor receptor gene in fresh human head and neck tumors","volume":"47","author":"Eisbruch A","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90592-7"},{"key":"e_1_2_1_20_2","first-page":"1648","article-title":"Growth inhibi tory effects of epidermal growth factor and overexpres sion of its receptors and human squannus cell carcino mas in culture","volume":"46","author":"Kamata N","year":"1986","journal-title":"Cancer Res"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fhed.2880110510","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.2880110510","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T09:43:31Z","timestamp":1697967811000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.2880110510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,9]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1989,9]]}},"alternative-id":["10.1002\/hed.2880110510"],"URL":"https:\/\/doi.org\/10.1002\/hed.2880110510","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,9]]}}}