{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:31:00Z","timestamp":1787859060704,"version":"build-2784847793"},"reference-count":9,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473419"],"award-info":[{"award-number":["82473419"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["07000044"],"award-info":[{"award-number":["07000044"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp;amp; Neck"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The aim of this study is to establish a novel and reproducible orthotopic mouse model, aiming to facilitate research related to laryngeal cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Implantation of mouse squamous cell carcinoma cell line, Meer\u2010LUC, into the larynx of C57BL\/6 mice (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200916). Tumor growth and survival were observed using magnetic resonance imaging and optical in\u00a0vivo three\u2010dimensional imaging. Laryngeal and cervical lymph node specimens were obtained for HE staining and evaluation. Single\u2010cell sequencing was performed on laryngeal cancer patients, orthotopic mouse models, and subcutaneous tumor models to investigate the immune microenvironment of laryngeal cancer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We successfully established 15 cases of in\u00a0situ laryngeal cancer mouse models. Tumors maintained steady growth at each observation time point, and histopathological evaluation confirmed the successful construction of the model. Single\u2010cell sequencing results suggest that the orthotopic model better simulates the immune microenvironment alterations of real laryngeal cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We have developed a novel and reproducible orthotopic mouse model, which more faithfully mimics the immune system response of the human body and better simulates the biological evolution process of tumors. This model provides an essential animal research framework for investigating the molecular mechanisms underlying the occurrence and development of laryngeal cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hed.70001","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T07:22:16Z","timestamp":1754896936000},"page":"106-113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A New Orthotopic Mouse Model of Laryngeal Squamous Cell Carcinoma"],"prefix":"10.1002","volume":"48","author":[{"given":"Junrong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital Central South University  Changsha China"},{"name":"Otolaryngology Major Disease Research Key Laboratory of Hunan Province  Changsha China"},{"name":"Clinical Research Center for Laryngopharyngeal and Voice Disorders in Hunan  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianguang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lunhua","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Animal Experimental Center Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1831-2737","authenticated-orcid":false,"given":"Hongxue","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Pathology Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xionghui","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erliang","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susheng","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery Harbin Medical University Cancer Hospital  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,11]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/JS9.0000000000000902"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-01031-z"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-019-0702-4"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006326203858"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003489414549575"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21386"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.000976"}],"container-title":["Head &amp; Neck"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.70001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/hed.70001","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/hed.70001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:22:51Z","timestamp":1782746571000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/hed.70001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,11]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/hed.70001"],"URL":"https:\/\/doi.org\/10.1002\/hed.70001","archive":["Portico"],"relation":{},"ISSN":["1043-3074","1097-0347"],"issn-type":[{"value":"1043-3074","type":"print"},{"value":"1097-0347","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,11]]},"assertion":[{"value":"2025-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}