{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T10:00:29Z","timestamp":1780999229881,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Head &amp; Neck"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Human papillomavirus\u2010positive (HPV+) head and neck squamous cell carcinoma (HNSCC) generally has a better prognosis, but some cases are aggressive with poor outcomes. This study established KUSCC\u2010192, a de novo HPV+ tonsillar cancer cell line, and evaluated its tumorigenicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>The HPV status in KUSCC\u2010192 was assessed using RT\u2010qPCR, RT\u2010PCR, and FISH. Aggressive characteristics were compared with other HPV+ cell lines and HPV\u2212 cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>KUSCC\u2010192 exhibited a mixed type with a predominantly diffused FISH pattern and a few dotted signals and lower E2 gene expression. These findings, combined with its aggressive features, suggest that the mixed type may contribute to the poor prognosis observed in 2%\u201330% of HPV+ HNSCC cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>KUSCC\u2010192 is a valuable model to study aggressive HPV+ cancer behaviors. Further research is needed to explore the clinical significance of mixed\u2010type HPV integration in HNSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/hed.70143","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T10:28:33Z","timestamp":1767090513000},"page":"1447-1460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>KUSCC<\/scp>\n                    \u2010192: A Novel Tonsillar Squamous Cell Carcinoma Cell Line With Mixed (Episomal and Integrated)\u2010Typed Human Papillomavirus From a Korean Patient With Tonsil Cancer"],"prefix":"10.1002","volume":"48","author":[{"given":"Jae Hyeok","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Research Institute of Medical Science, Konkuk University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Hwa","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Research Institute of Medical Science, Konkuk University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cha Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Research Institute of Medical Science, Konkuk University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Na","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Research Institute of Medical Science, Konkuk University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Chang","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Research Institute of Medical Science, Konkuk University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2020.104613"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20193"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541\u20107786.MCR\u201021\u20100831"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010023\u201002819\u2010y"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.008656"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24729"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41199\u2010018\u20100039\u20103"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14184488"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.26501"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21962"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30243"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29112"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1996.4596"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13027\u2010016\u20100107\u20104"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.31450"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.275911.121"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13164089"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2018.02.017"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878\u20100261.13219"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2018.12.008"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-23-0097"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2009.12.032"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.10837"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.164806.113"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12010042"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2009.02964.x"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2016.02.008"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14215213"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12535"}],"container-title":["Head &amp; 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